for this assay I find difficulty to assess the starting point of the reaction as absorbance of hydrogen peroxide with buffer is very small and when I added sample such as natural extract the reading is going to be high or may be overscale and when I diluted sample the reading can be detected but the reading is going to increase to point and then decrease and when incubation time is about 10 min decrease may be high at first but with time it may decrease again to give a small difference. the link attached here containing the method for this assay but in calculation the authors suppose that zero time reading is one but it was variable with my samples what method of calculation I should follow to detect scavenging activity in accurate way ....
http://www.biomedcentral.com/1472-6882/12/221
Thank you for your consideration
Regards
You can also read
Article Phytochemical Screening and Free-Radical Scavenging Activity...
Probably you can try the method as in doi:10.1016/j.foodchem.2004.10.006
or https://www.researchgate.net/profile/Charles_Lamien/publication/223275408_Determination_of_the_total_phenolic_flavonoid_and_proline_contents_in_Burkina_Fasan_honey_as_well_as_their_radical_scavenging_activity/links/00b49519cd5a9b15f8000000.pdf
https://bibliotecadigital.ipb.pt/bitstream/10198/2648/7/Edmur.pdf
Article Determination of the total phenolic, flavonoid and proline C...
Actually I did not find hydrogen peroxide scavenging activity of natural extract in the attached papers but it is ok. I already using the methods mentioned in papers
Thanks for consideration
Check these publications and links:
http://scholarsresearchlibrary.com/ABR-vol2-iss1/ABR-2011-2-1-127-131.pdf
http://journal.chemistrycentral.com/content/pdf/1752-153X-6-12.pdf
http://esa.ipb.pt/pdf/RefPlants_16.pdf
Check a similar thread and other questions on antioxidant property:
https://www.researchgate.net/post/Does_anyone_know_about_the_scavengers_of_hydroperoxide_radical?tpr_view=8284fb21-ac53-4c19-9993-dda0215b9268_3
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