What is the exact concentration and amount of acetosyringone required to achieve successful agrobacterium mediated gene transfer ?

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Vaccination is in its First phase in India. The front line is to be vaccinated yet. In this situation opening of institutes may be dangerous for the life of children.

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Some other person with similar name claim all of my research publication on research gate . how can I reclaim my Owen publication on this site?

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Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

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Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

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Chakravarthi Mohan

The acetosyringone concentration varies between plant species. Generally in our lab, we use 100 µM in rice and 200 µM for sugarcane. I suggest you to do a initial experiment to standardize the concentration for your plant of interest (or select based on articles).

Hassan Nima Habib

this link is useful

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4285021/

regards

Bhushan Bhadane

Thank you Chakravarthi Mohan and Hassan Nima...