We did an LBL assembly protocol, with PEI/PSS and PEI/PAA, on the surface of stainless steel needles - up to 6 bilayers. From experience and literature, this is known to be stable.
In the past, we usually store the prepared LBL-coated needles in +4•C, and still get acceptable rests.
However, a colleague mistakenly deposited the coated needles in a -20•C freezer, and they got frozen/frosted to a large extent. Right now, I’m unsure of going ahead with the downstream protocol, as I cannot ascertain the stability of the coatings. Any insights on that, based on experience, would be highly appreciated.
Also, I’d like to know how long, on the average, a thin film coating can last without losing it’s stability or structure. If you have any other storage ideas that work for you, I’d like to hear it please.
Thank you!
...and still get acceptable results**.
It was a typographical error up there please.
Dear Chukwumaobim Nwokwu, what is the glass transition temperature of this compounds? My Regards
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