What is the effect of the PUMA gene or protein, particularly it's role in apoptosis ?

More Essa Dirandeh's questions See All

In ELISA work, If I forgot to add stop solution. And I took the absorbance. Is there a way to adjust readings as if stop solution was added?

I have read somewhere that absorbance without stop solution is about half those with stop solution. Is this true?

14 March 2024 5,575 3 View

How to remove the preprint from researchgate?

Please, I urgently need to remove and delete this article preprint "Cytotoxicity and genotoxicity reveal the link between acute cadmium exposure and Alzheimer’s disease" from Researchgate. I can't...

13 March 2024 8,002 2 View

Hi, everyone is there any solution?

I am testing a horizontal wind turbine inside the test section of a wind tunnel. If I have a blockage ratio of about 19 percent, is it acceptable or not? If it is unacceptable, how do I calculate...

13 March 2024 9,284 0 View

How does AI affect our apprciation of poetry?

After digital poetry which initiated a new poetry that relies on technology in the creation of poem's structure and images, now we have what is called AI poetry. Within a few seconds ChatGPT app...

01 December 2023 1,919 2 View

Can loss be presented aesthetically through literature?

What does it mean that a poet or a writer expresses their experience of loss through art? is it an objective way of reconciling with the painful experience?

08 July 2023 1,540 15 View

Hi, everyone is there any solution?

I'm working on ansys CFX. Has any of you encountered this problem and is there a solution? Because I can't work on old files that I completed and I can't make new files? this is the error...

21 February 2023 305 3 View

Is self-praise a characteristic of good poetry in Arabic poetry?

In Arabic poetry, self-praise is a common idea, yet it is essential to think of the different purposes behind a poet's self-praise in each case as there are a number of factors (the literary age,...

24 December 2022 5,187 11 View

Is the poet's task to be descriptive or explanatory?

Should the poet, as an omniscient narrator in a novel, explain the meanings behind the images he is presenting or otherwise, should he only describe what he sees and leave the reader to conclude...

06 December 2022 7,499 6 View

Is the symbolist movement in modern literature a retreat to conventional ways of expression?

Traditional poets used symbols and so did the modernist poets, in the first case the interior of the poet's self is symbolized in the second (the modernist) the outside world is objectified and...

01 December 2022 4,671 8 View

Is the "subjective" in modern literature a form of romanticism in reverse?

Unlike the romantic attempts to be one with the world through imagination, modernist writers argue that one should abandon this independence of the ego and attempt to look at the world...

29 November 2022 5,088 4 View

Is this a facetotecta nauplius?

This larva was captured using a plankton net in the Persian Gulf during the summer. I believe it may be a Facetotecta nauplius.

08 August 2024 3,746 4 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

I need to dissolve stigmasterol for an animal experiment, it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent?

Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?

01 August 2024 7,504 2 View

CHO-K1 suspension adaptation protocol?

I am trying to adapt adherent CHO-K1 cells in F12K+10%FBS to suspension culture in only F12K, but in vain. I have followed Mirus bio as well as Thermo protocols, please suggest any alternative....

31 July 2024 3,756 0 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View

Are the apoptotic cells is positive for γH2AX ?

It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...

28 July 2024 7,983 2 View

Joachim Pimiskern

I've bookmarked these articles:

http://www.eurekalert.org/pub_releases/2010-07/waeh-mpr072810.php

http://www.eurekalert.org/pub_releases/2005-09/sjcr-ppc090805.php

The NewScientist seems to be offline currently.

http://www.newscientist.com/article/dn22296-block-egg-suicide-to-guard-fertility-after-cancer-therapy.html

Regards,

Joachim

Essa Dirandeh

Thanks Joachim,

Essa

Hi, thanks Lahiri