Azotobacter - PGPR Activities with Special Reference to Effect of Pesticides and Biodegradation

December 2015

DOI: 10.1007/978-81-322-2647-5_13

In book: Microbial Inoculants in Sustainable Agricultural ProductivityChapter: 13Publisher: SpringerEditors: DP. Singh

Hi dear Aniruddha Sarker , I congratulate you for this great work, its fun and practical topic and I hope that success will be with you.

Given that pesticide use is high in agricultural land, it is essential determination of the effect of these chemicals on PGP bacteria and their survival and functioning. For example, in the article " Impact of Pesticides on PGPR Activity of Azotobacter sp. Isolated from Pesticide Flooded Paddy Soils" the above has been addressed.

This article is available via the link below:

http://gjournals.org/GJAS/Publication/2014/May/HTML/010314003%20Chennappa%20et%20al.htm

Hi Mr. Sarkar,

Take take the median conc. of pesticide (final doze applied to field) and

I would suggest two method:

1. Zone of inhibition on plates

2. Microcosm study in the soil with same conc. and look for recover of microbes inoculated without pesticide.

As a PGPR you can take culture showing different PGP trait. Like (N-fixing: Azotobacter sp., Rhizobium, P_solublizing: Pseudomonas, Enterebacterace family bacteria, K-mobilizing bacteria, Biocontrol strain etc.)

Thanks Prof. Hayat Essalmani for your recommendation and doi link of related article. Thank you Mr. Bahman Khoshru for your article link as well. These are focusing the same theme as I think. Thanks for your kind contribution in this discussions.

Dear Dr. Kuldeep Patel thanks for your opinion. I already plan to set an experiment to isolate some pesticide degrader from soil by using selective media spiking that pesticide in agar plate. But, I also need to check their effect in soil, like bacterial diversity analysis, effect of metabolism etc.

Mr. Sarkar,

You can spike the pesticide in soil and also compare natural the soil respiration activity or autoclave soil and add spike PGRs and measure same. You can refer Thesis chapter 5, in following link, where instead of phytate you can add pesticide. Also same soil can be use to study the diversity of bacteria by isolating community DNA and use it downstream for DGGE or QPCR.

http://hdl.handle.net/10603/72057