I am wondering if the solvent has excitation and/or emission peak, does it affects the experiment. BTW, i already subtract the blank solvent spectrum from my sample spectrum. but i mean is the excitatio and fluorescence characteristics of solvent affect the accuracy of experiment??? Also, the intensity (y-axis) scale is up to 1000, so what is the maximum exc. and Fl. values of solvent should be??
The background can not deduct if the object gives intense fluorescence and does not give strong scattering. For objects with weak fluorescence or turbid solutions often have to subtract the scattered light.
Parasitic background occurs because due to non-ideality of the monochromator. For example, you excite the fluorescence in the region of 400 nm. However, the sample falls not only light with a wavelength of 400 nm, but a weak diffused light throughout the region 300-800 nm.
This light is recorded together with fluorescence and distorts the shape of the spectra.
If fluorescence is weak, and the scattering is large, the distortion of the spectra can be very large
If the solvent is fluorescing at the excitation wavelength, then it is also absorbing, greatly reducing the light available to your solute.
To check whether it is fluorescenc or scattering you have to use a different solvent that does not fluoresce
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