Can I use Hill's yield criterion, which is anisotropic, along with a Elastic Plastic Isotropic Model?

I am simulating a sheet metal forming process (Deformable Vs Deformable). Incorporating anisotropic elastic properties would need 21 constants. To make simulation easier, can isotropic elastic...

08 March 2020 3,693 4 View

Is there any algebraic formula to calculate thermal and frictional entropy generation for turbulent flow of nano fluid in annuli?

there is integral and differential method to calculate thermal and frictional entropy generation in in annuli. but i am looking for algebraic formulas as integral and differential method shields...

28 October 2019 2,564 2 View

Hello, how can i find a primer to identify Bacillus Sp. with PCR? Can someone help me with this?

I am looking for PCR primers for bacillus Sp.

15 February 2021 2,552 5 View

What could be the possible reason for the drastic variation in Qmax value for same adsorbent onto two different cationic dyes itself?

The adsorption capacity of the rice husk biochar was varying drastically with two cationic dyes namely Basic Blue 41 and Basic Red 09? What could be the possible reason behind this?

30 January 2021 1,420 3 View

What is the mechanism or interaction between Fe3O4 magnetic nanoparticles and biochar?

Lately there are many studies on biochar incorporated/loaded/coated with Fe3O4 magnetic nanoparticles. But I havent found any paper that describes the interaction or bonding between the two. How...

20 January 2021 3,162 4 View

the product size is 191bp would it be a problem during conventional pcr?

I have designed a primer for Bacillus subtilis sfp gene. As I have mentioned the size is 191 bp. however I need some suggestions regarding PCR temperatures and cycle length. As the it is smaller...

09 January 2021 8,309 4 View

Why does activation process called as activation? what does the term means actually?

I'm referring to activated carbon activation. Activation process does increase the surface area and functional groups content in activated carbon compared to biochar and raw biomass. But I'm...

30 December 2020 6,514 1 View

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Hi, I am interested to see the effect of BCG on macrophages (microglia )Therefore , I would like to know the amounts and the routes of administration which is safe in mice and how long is...

20 December 2020 7,166 2 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Best way for knockout of gene in Bacillus genome?

Hi everyone! I would like to knockout a gene in the genome of Bacillus. To do that I tried to make a construct that has 1) a backbone, 2) a kanamycin resistance cassette, flanked by 3) the...

24 November 2020 489 1 View

How can i get nonsporulating bacterial bacillus subtillis strain spoIIE without antibiotic growth conditions ?

10 November 2020 7,168 4 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View