Application of chitosan causes increase in activities of those enzymes which are involved in nitrogen metabolism and also improves the transportation of nitrogen in functional leaves leading to the enhanced plant growth and development.
Greetings!!!! Chitosan Acts as a plant growth factor and the mechanistic behind it is yet to be elucidated. But ya u can go for some parameters like NRA, CA and other photosynthetic parameters as they will give you a base from its physiological persona.
Please go through the following research articles for the detail answer of your query:
1. Gornik, K, M Grzesik and B R Duda (2008). The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. Journal of Fruit and Ornamental Plant Research 16:333-343.
2. Chibu, H, H Shibayama and S Arima (2002). Effects of chitosan application on the shoot growth of rice and soyabean. Japanese Journal of Crop Science 71: 206-211.
chitosan can provoke some enzymes involving the immunity system of plant and also some enzymes involving absorption of nutrients via root from soil. In addition, chitosan can fertile plant soil regarding microorganisms facet to facilitate release nutrients in soil and then their absorption.
Interesting subject, in strawberries sprayed with chitosan on the canopy at different times before harvest, we found a transient reduction of phytosynthesis that can likely to be ascribed to the film-forming activity (that in our studies amount for about 20-30% of its activity) and could be ascribed to reduction of gas exchange and/or reduced transpiration
Chitosan is considered a plant biostimulant. For instance, it triggers H2O2 accumulation and Ca2+ leakage into the cell. Both H2O2 and Ca2+ are signaling molecules that cause large physiological changes aimed at adaptation processes when plants are exposed to environmental stresses. They also can regulate developmental processes.
Chitosan is a safe and biodegradable plant stimulant. It is able to stimulate the enzyme production related to defensive responses in plants, such as catalase, peroxidase chitinases, glucanases and phenylalanine ammonia lyase (PAL) etc. It triggers the accumulation of different signaling molecules like Hydrogen peroxide, Nitric oxide (NO) which increase the activity of different defensive response by increasing the activity of defensive gene and increase the synthesis of plant secondary metabolites when plants are suffer from environmental stresses. Chitosan also increase the content of Jasmonic acid and ABA in plant resulting closure of the plant’s stomata and reduced transpiration during drought condition.