What is the easiest way to lyse sperm cells?

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Anderson Oliveira Souza

initially try to thermal shock...remove the samples of liquid nitrogen and transfer to bovine temperature 38 - 38.5°C

Aamir Javed

Dear Med ,

Heat Shock , Temperature Change ,Incubation Of Semen Sample @ Different Temperature,High Speed Centrifuge, Removal Of Seminal Plasma .

Some Time Inherent RBC and WBC Also Helps To Lyse .

Regards

Mohamed Nait Mouloud

thank you all for your answers,

dear Anderson

you're talking about rapid freeze-thawing method, isn't it?

How long should I leave the cells in liquid nitrogen / at 38°C?