I need a safe and easy protocol for the amylase activity in germinating seeds of wheat
dear dr.ujan you can contact to dr shahina remanullah associate professor in food science, university of Karachi
The 5,5'-Dithiobis 2-nitrobenzoic acid (DTNB) or Ellman's method is more robust and you would find methods in abundance for AA enzyme assay from any plant material ! best wishes.
It is possible to measure the starch content of an any embryo by spectroscopy method.
Kindly use a kit. Alpha amylase kit from megazyme
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