Hello everyone.
I inoculated 850 cells in 1Litre of water, but after 24 hrs I found no growth of bacteria. can anyone suggest what is the double time for s.aureus and at what time appoximately we can able to get the growth
https://pubs.asahq.org/anesthesiology/article/91/5/1394/39588/Growth-of-Staphylococcus-aureus-in-Diprivan-and
Missiakas DM, Schneewind O. Growth and laboratory maintenance of Staphylococcus aureus. Curr Protoc Microbiol. 2013 Feb;Chapter 9:Unit 9C.1.
The incidence and severity of infections are increased when Intralipid or Diprivan are administered to patients. Intralipid promotes infection, presumably by inhibiting the reticuloendothelial system, thereby suppressing the host's constitutive immunity, whereas Diprivan supposedly promotes infection by supporting bacterial growth and increasing the inoculating dose. This study considers whether bacterial replication alone in Intralipid and Diprivan adequately explains the increased risk of infection associated with these agents or whether other factors might also be involved.
i agree with Geis answer. Why water? Use a medium that supports growth. Doubling time and growth rate in general dependent upon the environment - the medium.
If the medium used is purified water, bacteria may not grow as it requires carbon, nitrogen, inorganic salts and growth factors for its growth. However, if the medium used is organic or contaminated water, it may grow with overnight incubation.
Dear Prachi,
as an inorganic chemist I'm certainly not a specialist in this field enough to provide you with a qualified answer to your interesting technical question. Thus all I can do right now is suggest to you a potentially useful research article. Please have a look at the following relevant literature reference:
Bacterial doubling time modulates the effects of opsonisation and available iron upon interactions between Staphylococcus aureus and human neutrophils
Article Bacterial doubling time modulates the effects of opsonisatio...
This article is not available as public full text on RG. However, the corresponding author has an RG profile. Thus there is a good chance that you can request the full text from him via RG at https://www.researchgate.net/profile/Michael-Brown-77.
Good luck with your research and best wishes!
Generation time is substrate-dependent and by extension on medium and environmental conditions
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