What is the dissociation constant for nicotinic acetylcholine receptors ?

More Bruno Cessac's questions See All

Can biogeographical regions work as a character in a character matrix for a phylogenetic analysis?

Basically I'm helping with the matrix using a cosmolitan group, could one of the characters used for be biogeographical regions (Neartic, Neotropical, etc)? Most of the data in this matrix refers...

25 July 2024 9,753 2 View

Dataset of synchronized cardiac angiography and ECG?

Hello, I'm working on medical project and I would need synchronized angiography with ECG? Does anyone know if some open source dataset of this kind exist? Regards, Bruno

25 July 2024 2,214 2 View

Cheaper alternative to XSens mocap system for ergonomic data acquisition?

Hello all, I'm starting a project where we want to automate the video analysis of people working in different environments to produce ergonomic measures like hip flexion, shoulder extension, etc....

16 June 2024 1,843 1 View

Why are my nanoliposomes turning purple??

Hello, I am making lipid nanoparticles using the thin film hydration method. Formula is DODMA:DSPC:Cholesterol:DSG-PEG2000 at 60:20:17.5:2.5 %mM in 5mM total. After hydration in sodium acetate...

16 June 2024 6,842 6 View

Isssues making a thin film of lipids for nanoliposomes?

Hello, Im trying to make a nanoliposome to encapsulate plasmid DNA. I am using the standard thin film method followed by resuspension in sodium acetate 25mM pH4 with my DNA (Im using the...

05 June 2024 3,646 3 View

How can I set the distance between peptide and bilayer using Packmol?

I still find Packmol very tricky with parameters. I have two pdb files, one with a pre-quilibrated bilayer and one with the peptide of interest. How can I create a system with three copies of the...

29 April 2024 8,231 1 View

Can you give me the new e-mail address of Sidney Gauthreaux?

Sid has retired from Clemson University, thus his address [email protected] is no more working,

28 April 2024 4,340 7 View

How to calculate the solute concentration in molecular dynamics simulation?

I am trying to calculate how many SDS molecules I need in a MD box to achieve a specific concentration (ex: 1M). My first doubt is if I use the box volume or the quantity of water molecules, for...

16 April 2024 2,051 2 View

Can someone indicates me a database about meteorological variables per country?

I'm looking for variables like solar irradiation, wind speed, humidity, temperature and precipitation

06 April 2024 1,926 2 View

How can I identify products of methyldopa oxidation by simple methods?

In alkaline solution (NaOH 0,1 M), methyldopa generates orangish solution. I suspect it turns into indolic compounds by base catalysed oxidation (possibly oligomers). How can I confirm...

01 April 2024 8,298 0 View

How to remove cell debris after tissue dissociation?

I'm facing issues with the tumor dissociation protocol for GBM. I use collagenase/dispase with DNase to dissociate my tumor sample. However, even after incubating it for 60mins, I do not get a...

22 July 2024 412 3 View

Does HEK293T cell express RIG1?

Is there any benefit in knocking out the interferon receptor in 293T cells? In the context of creating RNA viruses 📷

13 July 2024 3,382 0 View

Are there any people using biolegend anti- mouse CCR2-BV785?

Hi, I'm working on heart flow cytometry, and I use anti-mouse CCR2-BV785 (biolegend, cat150621) for staining, 20 minutes, 4 degree. But I couldn't see any CCR2+ cells. So I'm not sure if it's...

03 July 2024 3,969 4 View

How to find value for Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant?

Hello, I got reviver comment " please look into the comparative assessments regarding Ki (inhibition constant); Kd (dissociation constant), and Km "equilibrium constant" of the target...

09 June 2024 10,069 3 View

Is it hard to stain CCR2 for flow cytometry?

Hi, I'm working on the immune cells in the heart and have a problem with CCR2 marker. I did some research and found there's CCR2+ monocytes in heart failure mouse model. When I analysed CCR2...

08 June 2024 7,315 5 View

How to find the expected Kd value of a recombinant antibody?

We produced scFv of an antibody from bacteria. Now we want to establish dissociation Kd of antibody using quantitative elisa. How can i know the expected Kd value (range in nM, or mM)?

04 June 2024 2,602 5 View

How is it possible to model a protein that consists of several subunits using a Modeller?

Good day, I am trying to model the GABA A receptor, with different subunits. I selected the templates with the highest percent of identity, but as a result of the modeling I got a protein with...

02 June 2024 5,489 2 View

In order to compute frequency dependent dielectric constant using RPA ( with local fields ) in VASP how to set the NBANDS?

I am following these steps to calculate the dielectric constant of a zeolite. https://www.vasp.at/wiki/index.php/Dielectric_properties_of_SiC However, In step 2 IPA (independent particle...

02 June 2024 7,390 0 View

Need help making up 1L of 1% triton x-100 in 40mM sodium citrate?

At the risk of asking a stupid question. I'm planning to start isotropic fractionator experiments on insects. The protocol says to make a dissociation solution of 1L of 1% triton x-100 in 40mM...

27 May 2024 821 2 View

Conduction mechanism in dielectric in Jonscher power law?

In dielectric, how do we know whether conduction wrt applied frequency takes place due to electron or due to a polaron. In Jonscher power law, can we distinguish whether the conduction is due to...

16 May 2024 4,102 1 View

Peter D Maskell

What drug are you working with?

Bruno Cessac

Thank you Peter. In fact I am modelling Starburst Amacrine Cells activity and I would like to know if the Kd nicotinic acetylcholine receptors is known in this case, in natural conditions (without drugs).

Thanks

Bruno

As far as I am aware you need a ligand to have Kd.

Nicotinic receptors are cholinergic receptors that form ligand-gated ion channels in the plasma membranes. They can also be opened by nicotine.