I will coat an ELISA plate with capture antibody but I need to dilute these Ab in PBS or TBS. Can anyone tell me what is the different between the two? I know PBS is a phosphate buffer and TBS is a Tris buffer but what is the difference in using them as a buffer?
Hani Harb
well im not that expert but as i know that TBS has less salts in the whole buffer changing the ion-binding power of the antibody to the protein, whereas PBS has more salts making the binding somehow stronger. for the ELISAs i have always made i used PBS and never used TBS ever.
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