Raha Osilan

6 Questions 5 Answers 0 Followers

Questions related from Raha Osilan

How can I find the product length for the primers?

I am optimising a semi-quantitative PCR and I am testing alkaline phosphatase primers but I do know how can I get the product length of the primers , I Have tried to get it from pubmed but I am...

08 August 2015 6,660 10 View

Why do we clean coverslips with NAOH when we want to prepare polyacrylamide hydrogel?

I am preparing polyacrylamide hydrogel on glass coverslips and the first step is to clean the coverslip with NAOH to make surface hydrophobic, can anyone provide me a paper of that fact please?

10 October 2014 9,258 3 View

Can ascites fluid with capture antibody cause blocking in ELISA technique?

I want to measure Hsp60 in plasma samples but I am using monoclonal capture antibody with ascites fluid and diluted it in PBS but the assay did not work , and the optical density values were very...

07 July 2013 8,493 3 View

What is the different PBS and TBS in terms of using them as coating buffer in ELISA?

I will coat an ELISA plate with capture antibody but I need to dilute these Ab in PBS or TBS. Can anyone tell me what is the different between the two? I know PBS is a phosphate buffer and TBS is...

06 June 2013 8,362 1 View

What is the difference between Bicarbonate and PBS as a coating buffer in ELISA assay?

I am measuring Hsp 27 protein by sandwich ELISA and I will use monoclonal anti-Hsp 27 antibody and I need to dilute it in a buffer. The protocol says to use bicarbonate as a coating buffer....

06 June 2013 1,799 8 View

Can anyone remind me of freeze-thaw cycle please?

I need to measure the effect of freeze-thaw cycle on thioredoxin protein. As far as I remember I need to thaw samples at room temperature then re-freeze them again but do I need to aliquot them...

06 June 2013 7,481 1 View