Adil Khan Nested PCR like already everyone say its when you amplify first with a set of primer, and then use a second set of primer using of template the first pcr.
The semi nested method its basically the same, but instead of using a second set of primer totally diferent, you use one primer of the first set, and the second primer is inside the sequence you already amplify
Good luck!
Hello Khetam
For nested PCR you should be designing two primer pairs, one for the outer fragment and one for the inner fragment. The product of first PCR is the templete for the second PCR.
Have a nice day,
Ana Elvira
https://www.ncbi.nlm.nih.gov › NCBI › Literature › PubMed Central (PMC)
Good brief and simple differentiation between them ,I think it , by Dr.Ana Elvira
Thanks for all
The Polymerase Chain Reaction is a molecular biology technique to amplify a single or a few copies of a piece of DNA up to several orders of magnitude of a particular DNA sequence.
Nested Polymerase Chain Reaction is a modification of Polymerase chain reaction intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites.
http://resources.qiagenbioinformatics.com/manuals/clcgenomicsworkbench/650/Nested_PCR.html
Nested polymerase chain reaction (Nested PCR) is a modification of polymerase chain reaction intended to reduce non-specific binding in products due to the amplification of unexpected primer binding sites.
Polymerase chain reaction itself is the process used to amplify DNA samples, via a temperature-mediated DNA polymerase. The products can be used for sequencing or analysis, and this process is a key part of many genetics research laboratories, along with uses in DNA fingerprinting for forensics and other human genetic cases. Conventional PCR requires primers complementary to the termini of the target DNA. The amount of product from the PCR increases with the number of temperature cycles that the reaction is subjected to. A commonly occurring problem is primers binding to incorrect regions of the DNA, giving unexpected products. This problem becomes more likely with an increased number of cycles of PCR.
Nested polymerase chain reaction involves two sets of primers, used in two successive runs of polymerase chain reaction, the second set intended to amplify a secondary target within the first run product. This allows amplification for a low number of runs in the first round, limiting non-specific products. The second nested primer set should only amplify the intended product from the first round of amplification and not non-specific product. This allows running more total cycles while minimizing non-specific products. This is useful for very rare templates or PCR with high background
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