Hi all,
I found that in most of the papers, people are using the superior cervical ganglia from neonatal mice or rats instead of adult, for the culture of sympathetic neurons.
I would like to know what would be the reason for that ?
Are they any differences that we need to consider for the culture primary sympathetic neurons from SCG ?
Thanks
Dear Ibrahim,
I don't know about SCG in particular, but the usual reason for using neonatal neurons of the PNS is the ease of dissociating the ganglion (if that is what you plan to do). In adults, there will be a lot more connective tissue and support cells that are difficult to remove without damaging the neurons. Since there are only 2 SCG per animal, you want to maximize the number of viable neurons you get from your procedure. (There may be other reasons specific to the type of neuron also (neurotrophin dependence, for example); I am just not very familiar with the SCG.)
Cheers!
Jill
Jill's remarks are to the point. One gets better cell yield and higher numbers of 'healthy neurons from NN ganglion than from adults. Neonatal SCG neurons are also more viable and tolerate hypoxia better than adults. Check our papers for additional methodical details. Very best regards, Saak
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