Orbitrap Fusion FT keep getting disconnected?

Hi, I been having a problem with the Orbitrap Fusion for quite a while and it has been getting more and more frequent, which is very frusttrating. The fusion FT would get disconnected suddenly...

22 January 2021 1,235 4 View

What is the basis set to optimize ZnO using GaussView for DFT calculation study?

Greetings, I have a bit of problem in setup the basis set of my DFT study for ZnO.. As you can see in the image attached below, I use B3LYP 6-31G (d,p) for geometrical opt. of ZnO.. But sadly, I...

13 January 2021 7,508 4 View

How does titanium dioxide (TiO2) as a coating's component enhance the coating performance in terms of anticorrosion??

Greetings, Recently, I was asked by a researcher how does TiO2 enhance the coatings polymeric structure making it able to reduce corrosion reaction by preventing the penetration of aggressive...

02 January 2021 5,257 5 View

What are the suitable amplitude of sinusoidal perturbation signal to analyzed stainless steel 316L?

Greetings researchers, I want to ask for your suggestion in regards to what is the suitable amplitude of sinusoidal perturbation signal to analyzed the conditions of bare and coated stainless...

07 December 2020 7,699 7 View

How to improve DNA extraction using TRIzol and chloroform?

02 December 2020 1,919 1 View

What is the solubility of crude extract of plants in water?

Greetings all, To my limited understanding, the crude extract of plants obtained using solvents such as ethanol and methanol are usually insoluble in water. Is this correct? If it is soluble in...

02 December 2020 1,024 6 View

Does the concentration of the fluorescent compound affect the fluorescent reading on a 96-well plate?

I am trying to detect the fluorescent level of the extracted RNA sample on a 96-well plate. After extraction, I dilute the RNA sample in 20ul solvent. Can I load the 20ul RNA sample directly to...

30 November 2020 2,616 2 View

Any available international research grant in this COVID-19 year?

Dear researchers, I am a researcher from a university in Malaysia. I know that we all are having a tough situation in this pandemic year. I just posted this to know if there is any possible...

24 November 2020 8,759 3 View

In your opinion, what are the mechanism performed by corrosion inhibitor in a coating for corrosive medium?

Greetings researchers, There are quite a number of studies has been performed to analyze the effects of corrosion inhibitor when incorporated in a coating (epoxy, alkyd and etc) for the...

23 November 2020 6,622 10 View

For a marine coating, how much does its mechanical strength needs to be?

Greetings to all researchers, First and foremost, I would like thank all of you for answering this question. So, my question is quite general I guess, for a standard marine coating with good...

08 November 2020 8,880 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

Are there lab markers/pens resistant to Roti-Histol?

Hi, I'm looking for a pen/marker to write on the smooth, transparent (non-frosted) part of microscopy slides which does not get washed away by alcohols and most importantly Roti-Histol. I want to...

24 February 2021 9,902 5 View

I have a problem with kanamycin - why the kanamycin doesn't affect?

Hi everyone, I need to expression of GFP with pET28 plasmid in E. coli BL21(DE3) plysE. Transformed cells have kanamycin and chloramphenicol resistance. Normally, only transformed cells should...

22 February 2021 4,032 4 View

How does elongation affect the resistance of an electrode?

What are the testing rate for electrodes recommended by ASTM for cochlear implants. I am currently doing the 15mm/min elongatio testing.

22 February 2021 1,515 1 View

Resistivity of PVD TiNx thin films vs. N2 flow?

In the PVD of TiN thin films (~100A), I've found the resistivity of the TiN films dropped from ~100 to 60um*cm with N2 flow increasing up to the metallic mode ending point; then the resistivity...

21 February 2021 1,864 2 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

How to produce huge amount of protoplasts from Fusarium graminearum?

Hi, all I am using some protocol for protoplast production from Fusarium graminearum (Fg), but I need them in a huge amount. Sometimes the protocol worked well but most of the time failed....

14 February 2021 2,286 4 View

Recommendations on CO2 Resistant Rocking Shaker?

A certain protocol we have developed requires the use of a Rocker/Rocking Shaker in which our dishes with cells are maintained in the incubator. The rocking is essential to the protocol but we...

09 February 2021 3,291 1 View

Which is the most accepted taxonomy in Resistance to Change literature?

I am searching for a well accepted taxonomy of resistance to change and in terms of its underlying causes/roots.

04 February 2021 497 3 View