What is the difference between test wavelength and reference wavelength length of the cell viability assay?

Hend abdalla Alshabrawy @Hend-Alshabrawy

28 July 2020 0 7K Report

I have found that wst-1 assay has test wavelength 450nm and reference wavelength 630 nm, I am confused what is the difference?

Badges
Science topic

More Hend abdalla Alshabrawy's questions See All

What plants have high levels of sulfur-containing amino acids such as methionine?

Methionine, cysteine, homocysteine, and taurine are the 4 common sulfur-containing amino acids, but only the first 2 are incorporated into proteins. Sulfur belongs to the same group in the...

22 July 2024 4,273 4 View

How many cells are used usually for sphere assay (E0771 cell line ) ??

I have a problem with number of cells in E0771 cell line . I usually use 100-2000 cells for sphere but I couldn't get good sphere.

04 July 2023 1,099 6 View

What is the best test to compare continuous dependent variable between 2 groups of overlapping data?

I tried to use Mann-whitney test, but when i read the assumptions it shouldn't be overlap between the samples. Should I use Wilcoxon test ? But they are not the same study samples as well Thanks

13 August 2022 2,552 5 View

Is there any feature in the Microsoft word that help you making a more professional graphical abstract?

A graphical abstract is designed to help the reader quickly gain an overview of a research article. It is therefore imperative and adds value to your paper presentation. It will be good if there...

23 July 2022 7,096 6 View

What are the most recent effective drought stress resistance mechanisms applied to vegetable plants?

Water scarcity is the universal problem both in developing and developed countries. Numerous researchers have discussed different strategies to cope with this problem. I am looking for the recent...

19 April 2022 4,442 14 View

How does Zinc interact with Iron in plants?

Is there competitive interaction between Zinc (Zn) and Iron (Fe) in plants?? Can I add both in the same solution for the foliar application?

05 January 2022 8,195 4 View

How to treat plants with nanochitosan?

Nanomaterials including nano chitosan solubility is a common problem. It could be dissolved in 1% acetic acid, however, is this suitable to treat plants with ?

19 December 2021 4,448 4 View

Hemicellulose and lignin, which are more plentiful and more degradable by microbes in lignocellulosic materials??

Cellulose, hemicellulose, and lignin are three polymers. Both cellulose and hemicellulose are polysaccharides, however, lignin is an amorphous polymer that protects the cellulosic structure from...

12 November 2021 4,535 6 View

my citations on google scholar are increasing and so my H. index..but some of them not appeared on my RG account.why? any one has a solution???

any recommendations???

23 August 2021 8,248 13 View

What is the best software for microsatellite statistical analysis ?

I have made detection for microsatellite SSR markers in patient with plasmodium... for statistical analysis, I have used genalex 6.5 ... I still need to make confirmation with another...

14 July 2021 7,887 6 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View