Why can't I run sample more than 40 mins in Agilent HPLC even after loading method for 45 mins?

Hi, I am using an Agilent 1200 series and Chemstation software. When I try to set run-time more than 40 mins (for ex. 45 mins or more than that), run-time is getting over within 40 mins even after...

26 January 2021 7,136 3 View

Are there existing studies in economic sociology that have applied Kalyan Sanyal's theoretical framework as a methodological basis?

To be precise, I am looking for studies on informal labour that have taken on board Sanyal's category of the Need Economy and his 'Politics of Exclusion' in studying the political-economy of...

26 January 2021 4,219 3 View

How do we solve optimization problems involving a maxima or minima in the objective function ?

I am trying to solve a multi-objective optimization problem using the Pyomo library in Python. The objective function includes operational optimization of an electrolyzer for hydrogen production...

11 January 2021 8,018 3 View

I have weird declining basal OCR profile while doing a seahorse XFe mitostress experiment. Any suggestions on what is wrong here?

Hi all. I am doing some tests with transfections and getting this weird basal OCR profile in mitostress tests. Could someone tell me what is wrong here??? The cells look perfectly fine in wells...

30 November 2020 688 2 View

Physical significance of Gap between optical and acosutic branches in Phonon Dispersion?

Hello Everyone, Recently I am doing some Phonon branchesbased studies. For some materials the gap between the optical and acoustic branch is small, for some it's large and for some there is no...

04 November 2020 3,987 2 View

Anyone please suggest reference and scitation tool comaptible with WPS or Libreoffice?

Hello Researchers, I mostly use WPS and Libreoffice but the problem which is face several times is addinf references and citations. Because even when we change one of the reference it doesn't get...

23 April 2020 4,962 8 View

Why low sensitivity of MTT test for determining cell viability in fish cell lines?

While performing cell viability assay using MTT in fish cell lines grown in L-15 media without CO2 at 28 degrees, I found a highly faint purple colour in control cells (after solubilising formazan...

20 March 2020 8,108 3 View

Software required for Olympus microscope. Could anyone share the same?

Hello all, We have a Olympus DP71 model camera connected to an Olympus IX71 microscope. Unfortunately, the PC connected to it crashed and we lost the CD related to this software. The camera is...

10 November 2019 8,366 1 View

Master Thesis topics?

Hello All, I would like to do my master thesis on the following topics, but I am not sure how to choose it and also that matches with current market trend and my future job opportunities. Can you...

14 April 2019 7,028 4 View

How to test the change in a continuous variable over time between the intervention and the control group?

I am trying to see if the change in GFR (continuous variable) from baseline to 3 years later (total of two time points) is different between my control and intervention groups. The difference...

06 July 2017 5,134 4 View

Is there a deep reason for using the squared modulus of the wave function as the probability density of the existence of the particle?

I think we were all surprised at the first time we got to know quantum mechanics that the squared modulus of the wave function is the probability density of the existence of the particle? The role...

28 February 2021 6,282 9 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Quantifying leaking DNA from bacteria - what's the secret?

Hi, all! I need to determine if my treatment causes membrane damage in bacteria (S. aureus and E. cloacae), but I cannot use a fluorescent probe such as PI and similar ones because my compound is...

24 February 2021 2,006 2 View

How can I verify my centrifugal fan design for electric motor cooling?

I have radial blade centrifugal fan design which will be mounted on exhaust side of electric traction motor. The fan will be mounted on same motor shaft inside the housing. How can I verify that...

23 February 2021 2,390 3 View

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23 February 2021 4,014 3 View

Is there a suitable way to confine cell attachment area inside a multi-well plate?

Are there any types of cylindrical cell culture inserts that are designed to confine cell seeding area? Say, if I'm using a 12 well plate (3.5 cm^2/well), but only want to seed cells in the middle...

22 February 2021 7,127 4 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Hi..how to isolate crude HDL (all subfractions) from human serum by size exclusion chromatography?

I would like to know about isolation of crude HDL from serum by SEC. Is it possible to perform with a serum sample volume of

19 February 2021 5,576 2 View

Are ZnS nanoparticles moisture sensitive?

Hello Everyone, I tried to form ZnS nanoparticles using the hot injection method and was able to form the same (confirmed by XRD). When I centrifuged, after the reaction I got a kind of white...

17 February 2021 1,709 3 View