what is the difference between "input sample" and "ChIP sample"?? and, can i prove the binding of protein and promoter by performing only PCR and qPCR rather than sequencing and hybridization?
Please answer soon if possible.
Thanks.
Mustafa Safa Karagöz , Thanks. I was talking about chromatin immunoprecipitation (ChIP) and in literature survey I found the terms which are asked in the question.
Input sample is your full dna that start to work, and Chip sample the pull down DNA that attach to you protein of interest. Normally the signal in the Input sample is too strong, and should be diluted 1:10 or 1:100, is the reason why you can see in múltiple articles 10%input or similar. Input sample is used as a kind of positive control.
Regarding if you can do qpcr, yes of course if you are sure of the gene of interest that binds your protein its the easy way.
thank you so much Miguel Gallardo.
Another query is that when i start the ChIP from tissue, then i will get only the chip sample in the last step i.e. after immunoprecipitation. so how could i will get the input sample (full DNA)?
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