What's the concentration recommend for recombinant protein use in western blotting?

I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...

24 February 2021 8,514 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What can I do if DNA OD260/280 is either 1.6 or higher than 3?

26 January 2021 7,830 4 View

How do you multiple impute item scores and perform a linear regression on factor scores?

I am developing a questionnaire and first performing an exploratory factor analysis. After I have the final factor structure, I plan on regressing the factor scores on some demographic covariates....

18 January 2021 883 8 View

How do I design an extra long linker?

I'm trying to insert a linker between two proteins that is almost 100 amino acids in length, does anyone have any experience designing such a linker? I plan to order a gene block from IDT and...

17 December 2020 7,812 1 View

How to perform statistic analysis for age range between two cohorts?

Hi, I was reading the paper (MicroRNA Expression, Survival, and Response to Interferon in... ). In Table 1, I'm wondering how did the authors calculate a p-value of 0.29 for Age? The age in Table...

20 October 2020 2,276 5 View

Intron contetion and primer selection?

Dear all I am trying to clone a gene, which only contains only "one intron" in the middle part. I treat the extracted RNA with DNAse and use verso cDNA kit, so the DNA contamination ie...

10 September 2020 7,022 4 View

Does DTT affect cell lysis?

Hi, I am currently working with S. cerevisiae. general rule of thumbs for yeast cell lysis is to use 0.2M NaOH incubation followed by lysis using high urea buffer (containing 100mM DTT) at 70oC...

05 September 2020 6,915 3 View

Can we use pig imune serum to perform a passive immunization in mice?

We have vaccinated pig with antigens and then, we would like to know whether we can use mice to perform a passive protection assay by using the pig immune sera. Do you know pig macrophages can...

15 April 2020 2,510 2 View

Serum starvation before transfection and cell doubling time?

Hi, everyone I have a simple question about timespan of serum starvation and transfection. Recently I work on U-2OS cells which have doubling time around 20 hours, and before I transfect U-2OS...

15 March 2020 3,293 4 View

What are possible factors influencing the isotope-based hydrograph separation in river confluences?

When performing hydrograph separation (EMMA method) with isotope tracers in river confluences (natural large basin), we found that the contribution of each end member sometimes exceeds the...

20 January 2021 8,403 5 View

How to know if 87Sr/86Sr is reliable for Sr isotope chemostratigraphy in marbles?

I am currently looking for parameters to know if 87Sr/86Sr data is reliable for chemostratigrahy in marbles, and/or parameters to know if those marbles have had some post-depositional...

30 November 2020 9,032 8 View

Which isotopes can be used for preserving agricultural products?

Different kinds of Isotopes are playing very important roles in agriculture. They are used to detect different nutrient elements in soil and other crop improvement program. But which isotopes and...

16 November 2020 9,248 7 View

Dear fellows, what models are there available for water stable isotopes in the vadose zone?

I'm aware of Hydrus and Netpath, but are there others? I am highly interested in water stable isotopes in variably saturated and non-equilibrium flow (double porosity/permeability). Any hints...

21 October 2020 9,395 2 View

What are Standardization Parameters in Q and A ?

Q and A are parameters used for standardization and safety of drugs in pharmacology. Quality assurance is an integral part of traditional medicine, which certifies that it delivers the required...

06 September 2020 2,318 3 View

What is the difference in the experimental setup of gamma spectrometer for investigation of gamma attenuation and radioactivity check in soil samples?

will i require a collimator for investigation of gamma attenuation or the same experimental set up can be performed for both gamma attenuation and radioactivity check .I am using gamma ray...

29 June 2020 5,382 3 View

Are radioactive decay rates affected by temperature, pressure, or other environmental variables?

In my early education I was taught radioactive decay was - in general - not affected by environmental variables. This seems to have been disputed in recent years. I'm curious about the current...

22 June 2020 4,107 2 View

Have you published anything about the Ebrahimi meteorite? If so can you comment on anything pertaining to the isotopes of oxygen seen?

Did you see any evidence of D-ribose or other organic chemistry?

14 June 2020 9,565 8 View

What program/software can I use to search/mine a high resolution full scan mass spectrum (QTOF) for halogen isotope patterns?

For Bromine, the program would be able to go though each of the m/z's in the full scan, add 1.99795 (difference in mass between 79Br and 81Br) to that m/z, determine the intensity ratio between of...

16 May 2020 7,373 2 View

Does anyone has good references to "Organic x Inorganic CO2 in the atmosphere"? Volcanos x Burning fossil fuels?

I need more details and references about: the increase in carbon observed in the atmosphere is the organic origin CO2, not the inorganic origin CO2 released when a volcano erupts. Evaluation of...

19 March 2020 948 3 View