What is the difference between EndoC BH2 and BH3 cells?

31 January 2021 0 5K Report

I plan CRISPR experiment and I want to figure out what are the best cells for this. The plan is to use nucleation(electroporation)

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Reason for contradictory EdU and Ki-67 staining results?

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Why are there so many GC-MS peaks in my procedural blank when derivatizing with MTBSTFA?

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Are ABBA-BABA tests appropriate for use in a species complex with three introgressing groups?

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14 February 2017 6,263 2 View

How do I detect an antiantibody if this and secondary antibody are both raised in mouse?

As I want to detect antiantibody by ELISA, I coat the plate with purified mouse antibody(Antibody A). And then I add the serum from mouse which treated by Antibody A before. Finally, if I add Goat...

27 December 2015 9,709 7 View

What are the long-term impacts of incarceration on youths' developing brain?

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12 August 2024 862 0 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

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How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

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How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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What should Berlin do as a city to become as impactful as London and Paris in World Football?

Please go through my Abstract. I can also share a proposed Thesis Outline.

04 August 2024 2,077 0 View