09 September 2016 4 7K Report

I'd like to sequence a 600bp PCR product.

Here's my method:

1) PCR clean-up by QIAquick kit 

2) ligation with T4 ligase

3) transformation with heat shock

4) incubate on LB plate overnight

There's just  few colonies on plates, which step can I start with troubleshooting  ?

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