Diana Yang

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Questions related from Diana Yang

tomato genomic DNA digested with PstI ?

What can possibly go wrong in a simple PstI digest? reaction component : DNA(50ng/uL) 4 uL 10XNEBuffer3.1 1.2 PstI(20u/uL) 0.4 ddH2O 6.4 ----- 1) DNA...

03 March 2018 6,751 5 View

How can I extract plant DNA in a quick way?

Is there anyone have quick DNA extraction protocol? I use maize as my plant material, and extract DNA by Epicentre' quick extraction kit. But this item is stopped selling now. I am trying to...

03 March 2017 6,312 26 View

What is the difference between competent cell ''DH5-alpha'' and ''top ten''?

I'd like to sequence a 600bp PCR product. Here's my method: 1) PCR clean-up by QIAquick kit 2) ligation with T4 ligase 3) transformation with heat shock 4) incubate on LB plate overnight There's...

09 September 2016 7,419 4 View