What is the difference among promoter for multiplex genome editing

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Uncallable SNP showed significant to MTA, what to do?

I am getting a significant marker trait association for traits. But understanding the allelic association is difficult; NN is the missing allele. Can anyone help me?

13 May 2024 8,860 2 View

Why negative dielectric loss is coming at lower frequency range i.e from 4 to ~100 Hz?

I have performed the temperature dependent dielectric measurements;with proper checking of all electrical connections of electrodes, sensors,heater etc.as well as their proper resistance of...

07 April 2024 7,625 1 View

Which books one should follow to know details about Minkowski functional and its geometry in Banach spaces?

I am looking forward to know the concept of Minkowski functional and its geometry to construct a semi-norm? Can anybody suggest me with some good soft copies?

05 March 2024 5,234 2 View

I am trying to extract RNA from Streptomyces sp by manual method I am unable to get any results Kindly help me to find a standardized manual protocol?

Please send me some standardized manual protocols for the extraction of RNA from Streptomyces sp. Thank you in advance.

18 February 2024 7,298 2 View

What are the experiments I can include to study protein-protein interaction in vivo in mouse?

I want to design experiments to study protein-protein interaction in mice.

21 January 2024 9,893 0 View

What might be a possible reason for a drug to show efficacy when administered orally in humans but not in murine?

A drug showing efficacy in humans when administered through oral route, but the same drug is lacking efficacy in mice admistered through same route. What can be the possible reason?

08 January 2024 4,189 3 View

How to write a good research paper to publish in reputed journals like SCOPUS on my research area?

The background of my research topic is : I am doing research on a "community tourism" project which started in the year 2016 by the state government of Odisha. Odisha is one of the states in...

18 November 2023 1,292 2 View

How are the shells useful in core@shell nanostructures for energy storage purposes? Can thickness of shell play a vital role for storing energy?

How are the shells useful in core@shell nanostructures for energy storage purposes? Can thickness of shell play a vital role for storing energy? Is there any relations between thickness of outer...

16 October 2023 9,275 1 View

The high dielectic constant materials merge with low dielectric constant materials for energy storage purposes. WHY?

Some polymers having low dielectric constant used with high dielectric ceramics. But why we are merging these two high and low dielectric materials?

16 October 2023 2,170 3 View

Is it necessary to changes negative meaning senteces into positive; after doing reverse coding of a 5 point likert scale responses in SPSS?

I have collected data through survey from in 5 point likert scale where 1 as Strongly Disagree to 5 as Strongly Agree. I have total 20 questions in 4 constructs: economy, psychology, political...

08 October 2023 8,723 8 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Why my colony PCR results of my recombinant bacterial not showing any results?

I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....

05 August 2024 2,570 3 View

Who of all the Global Scientific community will help me Prof. Dr. Yoshida make way for TPEOM, MEC ~EMC to return the atmospheric gases to the norma ?

TEP presentation caption (The Environmental Project) Re: Why should Washington’s DC, or any country government point of location think of as nowadays of as to being 'tomorrow as to come! if it...

03 August 2024 2,484 1 View

How is the bacterial genome's high protein count verified as genuine despite 800+ contigs and good metrics (98.55%completeness, 0.68% contamination)?

Given that the bacterial genome has over 800 contigs, but its quality metrics are good, with a completeness of 98.55% and a contamination of 0.68% as assessed by CheckM, what specific validation...

01 August 2024 1,514 1 View

Can we use technical replicates for statistical analysis of multiplex cytokine data?

I have done an experiment using luminex technique, measuring 46 analytes. but my sample size is less (N=6, for case and control each). However, i have put up the experimental set in duplicates....

31 July 2024 3,665 3 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View

Inquiry on Maximum Nucleic Acid Volume for 2.5 mL Liposome Solution?

I am currently working on a project involving liposomes and need to determine the maximum volume of siRNA that can be added to a 2.5 mL liposome solution with a total lipid concentration of 10...

30 July 2024 6,420 1 View

Recovery Viurses from bacteria genome?

Hello everyone, I am currently looking for tools to recovery viral genomes from bacterial genomes, not metagenomes. However, I have only found tools that are designed for retrieving and studying...

28 July 2024 8,953 1 View

Are these cassettes suitable for expressing PETase mutant in E. coli?

I created two potential gene expression cassettes (constitutive and inducible) for expression of a mutant PETase gene on PeptiCloud using the version tree feature, which allows users to create...

28 July 2024 7,559 1 View