How to interpret different leached phases of metapelites?

Extraction of three different phases of metapelites, mostly phyllites as the Carbonate phase, Fe-Mn Phase, and Silicate phase has been obtained. After doing ICPMS, different results have evolved....

11 February 2021 5,198 10 View

Is anyone working on measurement of globalization?

I am in need of the help of anyone who is working on measurement of globalization? Please inform me at the earliest.

23 January 2021 1,259 1 View

Whether new variant will affect the diagnostic method of detection or not?

can it be diagnosied by the same tool or different tool

04 January 2021 2,964 2 View

Python modules for old Spectrum-Analyzers?

Hi All, Are there any python modules available for old HP-Agilent spectrum analyzers around? I know PyMeasure has a library of some commonly used and new electronics but I am looking for old ones...

21 December 2020 3,460 3 View

What should be the ideal voltage and current to scan mice femur and tibia using micro CT instrument ? How these parameters affect the image quality ?

I want to scan my experimental mice bone samples using micro CT instrument. I have gone through several research articles for ideal scanning parameters like voltage, current, filter etc. but these...

11 October 2020 8,069 2 View

How to calculate the defect charge formation energy for a metallic system (bulk/surface)?

How to calculate the defect charge formation energy for a metallic system (bulk/surface) using DFT? The defect formation energy is calculated by the following formula E_formation (D,q) = E (D,q)...

23 August 2020 6,047 4 View

What is form factor of a compact antenna?

How to determine the form factor of a miniaturized antenna?

18 February 2020 882 3 View

What does it mean by energy band convergence in semiconductor by doping?

If band structure is anisotropic in k-space, then how will the convergence of two bands normally appearing at two different symmetric point in the Brillouin zone will enhance See-back Coefficient?

04 February 2020 1,916 3 View

What is the standard procedure of seaweed sample preparation for SDS-PAGE?

Hi everyone... For SDS-PAGE sample preparation,I used 250 microlitres of a seaweed sample with 250 microlitres of treatment buffer. But I didn't get any band/protein fraction in it. So can anybody...

29 January 2020 633 2 View

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06 September 2019 8,466 10 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View

Which CpGs have a significant effect on gene expression?

It is known that methylated CpG around TSS decreases gene expression. However, It is difficult to know exactly which CpG has a greater impact on gene expression. Are there any databases known...

20 February 2021 5,634 3 View

Found that there was a multiple band when amplifiying targeted gene ?

i few days ago amplified specific gene using PCR with specific primers, but there was a multiple band in electrophoregram i found. out of 10 sample amplified, there was multiple band and there was...

18 February 2021 5,131 5 View

Why is there no protein expression but antibiotic resistance in transduced A549 cells with pLVX-TetOne-Puro lentivirus?

Hi, I have been trying to transduce A549 and Calu-3 cells using the Lenti-X Tet-One inducible expression system (TakaraBio). My gene of interest is inserted in a pLVX-TetOne-Puro plasmid and has...

15 February 2021 8,462 3 View

How many days do we need to detect adoptively transferred Treg in the tumor site in tumor-bearing recipient mice?

Hello, I've got a plan to isolate mouse splenic Treg (WT and KO of target gene) and inject these CD45.2 Treg into tumor-bearing CD45.1 mice. My question is how many days do I need to wait to see...

11 February 2021 8,238 1 View