What is the chromatographic technique used for the Gentamicin quantification?

HI everyone, I want to quantify the gentamicin antibiotic presenting in the culture medium. Can You anybody please suggest me suitable chromatographic technique for that? Please suggest me the...

16 December 2020 9,823 3 View

Principal Component Analysis?

Hi everyone, I want to perform Principal component analysis (PCA) for Excitation Emission Matrix (EEM). Can You anyone give the python language coding for it?...

17 November 2020 2,776 5 View

How do we interpret the following gender differentiated data?

Hi, We are analyzing data and have found the following finding. The results show that men and women hold equal responsibilities for many aspects of forest management and decision making in the...

27 October 2020 6,399 4 View

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation?

Can someone help me with different watershed management practices for hilly areas ? Requirement is to have enough water for crop cultivation. I am looking at the hilly areas, any proof of concept...

05 October 2020 5,641 15 View

Can anyone suggest from where I can get Microgrid data, such as nodes load, impedance etc ?

I'm working on Microgrid load management.

17 October 2016 8,272 5 View

Project Inputs

Hi, I am doing Ph.D in IT and specialized in Information Secuirty. My current project is Artificial Intelligence in Export System. I am expecting some inputs from your. So kindly help me. RS...

05 November 2010 5,483 0 View

What is meant by atmospheric river?

my project is based on atmospheric water so,i think it will be helpful for me.if i know about this

01 January 1970 6,112 5 View

Ethnobotany Theory - titles that further critical thinking and the development of the discipline of ethnobotany

01 January 1970 900 7 View

A new package EthnobotanyR

01 January 1970 2,734 22 View

In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to calculate the annealing temperature of a primer pair?

I have to amplify a gene and my primers just reached. The Tm for Forward primer is 64.2, and that of reverse primer is 65.5. Can some one suggest how to get the best annealing temperature? Thanks...

01 March 2021 360 7 View

Anybody with an idea on how do design specific primers for detecting tomato resistance genes tm-1, TM-2 and Tm2^2?

I am trying to identify these 3 genes among some tomato cultivar collections and after aligning some sequences from NCBI, I couldn't find unique sequences to target for specific primers. There...

28 February 2021 606 3 View

What is the ideal efficiency for a qPCR?

hello everyone, I need to do standard curves for my qPCR, what is the ideal efficiency range? I tried a primer (Mglu2 receptor) that gave an efficiency of 90.2%. Is it accepted?

28 February 2021 1,254 3 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Genescan gives 3 peaks instead of one?

Hi all, I am doing a genescan analysis for a deleted exon in the patient sample. The amplified region is 215 bp. In the patient sample because of homozygous deletion, no peak is observed while in...

23 February 2021 5,645 1 View