What is the amplicon size of 16s universal primer (63F and 1387R) and (27F and 1492R)?

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Anybody have proficiency in openLCA software here?

Hi, I want to do Life cycle analysis with openLCA software. can you anybody tell me how to create a product system with openLCA?

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Base hydrolysis of 2,7 dichlorodihydrofluorescein diacetate?

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Where can I get ethylene propylene diene monomer rubber (EPDM) standard refence materials?

I trying to analyze EPDM using pyrolysis gcms. Does anyone know where I can get the standard reference materials?

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What I must do if my isolate is 99% similar to Unclassified microalgae ?

Recently, we identified our microalgae using 18s rRNA, and the data showed that the isolate was 99% similar to unclassified microalgae with general information on NCBI and no publications. Our...

08 October 2022 6,624 3 View

Can you anybody suggest me a good tutorial to learn KEGG Pathway analysis?

Hi everyone, I want to learn metabolic pathway analysis from basics. Please suggest me good Youtube and text page tutorials. Thanks in advance

02 August 2022 8,176 4 View

What does Indicator Species Analysis (ISA) illustrate?

What does Indicator Species Analysis (ISA) illustrate? As I understand, indicator species, are species that are vulnerable to ecosystem perturbation. Even minor changes in the environment will...

08 May 2022 9,508 4 View

Can I conducte a Bivariate Pearson's Correlation test with factors/variables taken from a previous Factor Analysis?

Hello, I would like to know if I can conducte a Bivariate Pearson's Correlation test with factors/variables taken from a previous Factor Analysis? The idea would be to investigate the...

17 April 2022 3,193 5 View

Why do bioflocculants fail to flocculate real waste water?

Hi everyone, I extracted a bioflocculant from a Klebsiella sps. (isolated from activated sludge). That flocculant showed good flocculation in kaolin suspension with the aid of...

30 November 2021 9,966 8 View

What is the chromatographic technique used for the Gentamicin quantification?

HI everyone, I want to quantify the gentamicin antibiotic presenting in the culture medium. Can You anybody please suggest me suitable chromatographic technique for that? Please suggest me the...

16 December 2020 9,988 3 View

Principal Component Analysis?

Hi everyone, I want to perform Principal component analysis (PCA) for Excitation Emission Matrix (EEM). Can You anyone give the python language coding for it?...

17 November 2020 2,918 5 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View

Do you mix and add all primers together in a singel PCR reaction (4 total primers for 2 mutations) when using Agilent's multi-site mutagenesis kit?

I want to introduce 2 mutations using Agilent's multi-site mutagenesis kit, I designed the primers using their online tool and it gave me 2 sets for primers (1 set for each mutation) so I was...

25 July 2024 6,517 3 View

Can I please ask why my samples from anaerobic bioreactor giving me different size PCR product even after multiple runs?

Hi everyone, I have extracted DNA from a biogas bioreactor using Qiagen kit and prep cDNA library then used this library as template to optimize primers for qPCR (taken from papers). Some of the...

23 July 2024 1,329 5 View

Are there always been barcodes, apapters and primer sequences in the FASTQ files of NGS?

Hello researchers, Sorry for my stupid question. I am learning the QIIME2 workflow for analyzing some 16s amplicon NGS fastq data. I found a very nice paper with data and code public available...

20 July 2024 5,405 2 View