What is the definition of DS in primer sequence?

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How to completely dissolve soy lecithin?

Hello, I'm looking to dissolve soy lecithin in order to create a liposome. I've tried using various solvents like pure ethanol, methanol, and a mixture of ethanol and methanol but they didn't...

02 August 2024 6,980 4 View

When using NVivo software for data analysis, is it necessary for the researcher to also manually analyze the data?

it's my first time using NVivo software.

01 August 2024 9,304 5 View

How to convert a potential collected with reference to Saturated calomel electrode to Reversible Hydrogen Electrode?

I have collect a LSV data by taking SCE as the reference electrode. But I want to convert this one to Potential with respect to Reversible Hydrogen Electrode. Please suggest me the required...

13 July 2024 7,841 2 View

Regarding solubility of Dorsomorphin in DMSO ?

I'm using Dorsomorphin (C24H25N5O) as one component in dual SMAD inhibition of pluripotent stem cells to differentiate into neurons alongwith SB431542. Now as to solubilizing 10mg SB in DMSO for...

11 July 2024 4,832 0 View

Is it a source for moisture factor in different plants?

Is it a source for moisture factor in different plants? I need to find moisture factor (oven dry weight / wet weight) in plants in different studies.

10 June 2024 842 0 View

How can I clean autoclave (PTFE) from sulfur contamination ? in all aspect of color, smell and existence of Nano particle of sulfur??

the autoclave has been smelly and seem likely yellow after my synthesis and this changes may effected on other synthesis, so I want to clean PTFE from all sulfur contamination that remain on...

31 May 2024 2,985 0 View

What's the optimal temperature for freezing a lipopeptide solution before lyophilization?

I have a lipopeptide solution that I intend to lyophilize in a few days. Should I put it in the -20 freezer or -80? Which temperature is best for maintaining its properties?

20 May 2024 3,893 0 View

Challenges in Identifying Crack Initiation and Interpreting Results in Abaqus Direct Cyclic Fatigue Simulation?

Dear researchers, I'm currently conducting a direct cyclic fatigue simulation in Abaqus, but I'm encountering difficulties in analyzing the results. Despite the simulation running, I'm struggling...

04 May 2024 6,957 2 View

Cloudy background of polyacrylamide gel after de staining, a confusing problem?

Hello. My polyacrylamide gel becomes cloudy after de staining with a solution consist of (50% miliQ water + 40% methanol + 10% acetic acid) and the background of my gel does not become completely...

02 May 2024 895 2 View

Fail OCR measurement for my ReN differentiated cells?

I seeded 30000 ReN progenitor cell on XF96 plate which coated with matrigel. I differentiate them for 2 weeks and read OCR with inhibitors. However nothing worked. FCCP and oligomycin were same...

17 April 2024 9,012 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

After performing symmetric PCR, PCR purification was performed. Afterwards, asymmetric PCR was performed using the PCR purification product as a template, but no ssDNA band was confirmed in the...

08 August 2024 1,668 3 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Why after performing site directed mutagenesis ,I don't see any colony after transformation?

I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...

05 August 2024 9,059 3 View

Anyone having idea about VN primer for miRNA primer design ?

How to design VN primer to attach with universal reverse primer

05 August 2024 2,116 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

I'm optimizing a tetra-ARMS PCR protocol with amplicon sizes: 165bp, 123bp and 93bp. Why, in the gel, only 165bp is visible while I'm expecting all 3?

It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...

01 August 2024 4,673 4 View

PCR showing no bands - Master mix and primers didn't mix?

Hello everyone, I performed a PCR yesterday, and the results showed no bands on the gel. Of course, I probably missed some crucial steps, like adding my samples to the PCR strips themselves, for...

31 July 2024 2,406 6 View

Dimensions of an MJ Research 96-well alpha module?

Can anyone with an MJ research / BioRad PCR machine from ~2010 or earlier tell me the external measurements (LxWxH) of the removable standard PCR alpha modules that can be removed from a PTC200 or...

30 July 2024 2,867 0 View

How to retain amplicon yield after Ampure bead cleanup, following a 2-stage PCR protocol?

Hello all, I have been trying to follow a 2-stage PCR protocol used to amplify barcodes of a large yeast library, as per Nyugen et al. (2022) -...

30 July 2024 841 2 View