Can you suggest some plant transformation vectors that I can use for the expression of GFP/YFP-tagged protein of my interest?

I want to generate stable Arabidopsis transgenic lines expressing my protein of interest fused with GFP/YFP under the control of its native promoter. I want this for localization studies. Please...

23 January 2021 2,790 3 View

Queries regarding Antibody labelling ?

Hello Everyone. I was trying to label antibody with Cat# MO-L007 - Cystein reactive monolith series antibody labelling kit. I followed every steps that the protocol states. But in the final step...

26 September 2020 3,439 1 View

How do we calculate mechanical stress using VASP Calculations for Ab Initio Molecular Dynamics?

If we want to uniaxially strain a structure incrementally, and calculate the corresponding uniaxial stress at every strain point, how should we use the VASP output files for this purpose? I have...

04 April 2020 9,747 4 View

How do you model CO2 on VASP?

I am new to VASP, and I'm trying to model CO2. I was able to run the simulation and got all the output files, which I uploaded onto VESTA. However, VESTA shows that the bond length between the C...

23 January 2020 8,919 4 View

Can I use gromacs order to compute tetrahedral order parameter of water?

I want to calculate Orientational tetrahedral order parameter of water. The output of gmx_hydorder is confusing. can I use gmx_order instead?

17 January 2020 6,623 4 View

Cross section test to see whether second generation unit root test is required or not ?

I have a confusion. I had considered data in context of a particular nation in a paper and a reviewer suggested me to go for second generation unit root test instead of first generation unit root...

24 December 2019 9,239 1 View

How to determine the box dimension for umbrella sampling?

I am using Gromacs 2016 to do umbrella sampling of a protein-RNA complex. I am following the Lemkul's tutorial of umbrella sampling, but I couldn't get how the box dimension was determined. For a...

24 April 2019 1,458 7 View

How to convert Rice gene IDs to NCBI gene IDs ?

For scientists working in rice, is there any converter/annotation file to convert RAP DB/ MSU IDs to NCBI gene IDs ?

03 April 2019 3,324 4 View

What model should be used for exafs data fitting of hydroxyapatite?

I am trying to fit Sr K edge exafs data of Sr-substituted hydroxyapatite. I want to know about the choice of fitting parameters for the scattering paths. In particular, should I choose atom and...

11 February 2019 6,065 6 View

I want to show the binding between Pol-l-lysine and glucose oxidase or any other similar type enzyme using two fluorescent dyes. Dye for tagging?

FITC-TRITC protocol when both used together? any other florescent dye for tagging?

23 November 2018 1,944 3 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Resistor definition in Piezoelectric bimorph with ANSYS workbench

Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,

02 March 2021 0 0 View