Hi Nikhil,

That depends on PCR efficiency. Actually, when you search the internet a bit, you will immediately come up with some good search hits that will explain you the relation between the slope of a standard curve (made by e.g. 10-fold dilution of standards) and the PCR efficiency.

Examples: when PCR efficiency is 100% the slope should be -3.3 (cycles per 10x higher concentration). When efficiency would be around 90% the slope would be -3.6.

For more information search the internet for e.g.: PCR efficiency, real-time PCR standards.

Best,

Stephan

Hi Nikhil,

That depends on PCR efficiency. Actually, when you search the internet a bit, you will immediately come up with some good search hits that will explain you the relation between the slope of a standard curve (made by e.g. 10-fold dilution of standards) and the PCR efficiency.

Examples: when PCR efficiency is 100% the slope should be -3.3 (cycles per 10x higher concentration). When efficiency would be around 90% the slope would be -3.6.

For more information search the internet for e.g.: PCR efficiency, real-time PCR standards.

Best,

Stephan

As Stephan says, it's about 3.3 cycles.

Remember PCR is a doubling process, so a ten fold dilution should be around 3.3 cycles (2^3.3=9.48).