The peptides I'm working with are very likely to attach on the wall of the vial I'm using for LC-MS/MS. I saw many people suggested to use 2-3% BSA as carrier in the solvent for peptide/protein dilution. But the BSA (2.5%, 0.5g in 20 mL 0.1% formic acid) looks very concentrated, since it forms lots of foams during vortex. Could anyone tell me if this is OK to use or should I try lower concentrations? I appreciate any suggestions.
We have found the concept of a Non-Proteome Reference Protein (NPRP) quite useful. Much more efficient than BSA at far lower concentrations. By adding a surplus of a protein wich is almost identical to the analyte, adsorbtion can be effectively supressed. It is a similar effect as found when adding stable-isotope labelled versions of the analyte as internal standard - it modifies the chemistry also.
DOI: 10.3109/00365513.2014.917697
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