What is the standard procedure for disposal of ethanol? Can it be drained down the sink?

Can individual labs formulate their own procedure for disposal of chemicals or is there a universally recognized system of procedures for disposal of chemicals?

01 February 2016 4,129 3 View

Best tool for comparing multiple DNA sequence alignments?

Is there any nice online tool that can be used to evaluate different MSAs so we can decide which is the best alignment tool for a given set of nucleotides sequences.

31 December 2013 4,077 10 View

Best tool to build a phylogenetic tree for uncommon models?

This specially relates the models used in jmodeltest2, these models are not commonly encountered in common programs like MEGA etc. For example jmodeltest2 predicted 012030+G+F as the best model...

10 November 2013 3,962 4 View

Does anyone have an idea what this micro-organism could be?

The image is of fresh unstained culture under oil immersion. The other image is that of its colonies on LB agar.

06 July 2013 6,757 0 View

Can anyone suggest primers sequences for targeting 16s rDNA signature region of Bacteria in general?

I want to confirm presence/absence of endosymbionts within Acanthamoeba cells and for that reason I need generalized primers that can target the bacteria DNA. If positive I would later get it...

05 June 2013 6,376 3 View

How crucial could the quality of agar be for growth of bacteria (Arcobacter in particular)?

I was growing my Arcobacter happily using the recipe for Vandamme media (broth and agar). Then suddenly I had either poor growth or not growth in VD agar. All the ingredients were the same except...

04 May 2013 3,952 0 View

Can anyone suggest list of antibiotics which dont penetrate through cell membrane and therefore can be used for killing extracellular bacteria only?

Such antibiotics are used for killing extracellular bacteria during antibiotic protection assay for studying the intracellular survival of bacteria inside cells (macrophages etc). Gentamicin,...

11 December 2012 9,063 10 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

No title

22 February 2021 9,932 11 View

Is it okay to mention you distributed your qualitrics study link through social media sites?

Just want to be clear and know it is okay to say I have distributed the qualitrics link through social media such as Facebook, Instagram and Twitter to ensure my target audience is reached in my...

21 February 2021 5,143 2 View

What is the minimum number of exosomes/ml to have a good yield of total RNA for Illumina sequencing?

I am using IZON qEV2 columns to isolate exosomes from 100 ml of cancer cell conditioned medium. in NTA analysis I usually see concentration of 2x10^9 particles/ml. But when I proceed with this...

20 February 2021 6,879 3 View

Any suggestions or recommendations on a good publishing house based on the African for academic books in the field of Communication and media studies?

I am currently working on a book on Public Relations (from an African perspective) and the rationale behind the book is the fact that as instructors in this field, we borrow instances and case...

19 February 2021 2,482 2 View

Are there papers on?

I'm looking for a papers concerning either Christianity or neopagan religions and social media. What role does social media play in the spread of these religious movements? Any suggestions on the...

15 February 2021 655 4 View

Any probe or assay for the detection of Cysteine in yeast invivo ?

Dear fellow researchers, I would like to detect the cysteine levels for yeast in vivo. I would really appreciate if anyone could suggest me any fluorescent probe or assay kit available. Currently...

11 February 2021 3,292 3 View

A film coverage that allows for transmittance of wavelengths on the infrared scale?

I've landed on needing some type of highly optically transparent film or cover material that prevents evaporation of culture media; I am not sure which subfield of materials science would be able...

08 February 2021 5,760 1 View

Which fungicide are good for primary cells? Does it yeast contamination? How to get rid of them without killing the cells? How to get rid of spores?

Hello! Our lab is working with primary cells which we are isolating from the colorectal tissue. During the isolation, we are washing the tissue with pen-strep and then also add some pen-strep in...

07 February 2021 8,258 3 View

Best analysis method to use for my variables?

I need to conduct an analysis and wanted to check if my idea for analysis sounds feasible. I have 3 DVs which are; fear of terrorism, confidence in the police and punitive attitudes towards...

07 February 2021 5,746 10 View