How to judge and prove the uniqueness of the solutions to the following two matrix equations?

It is about the matrix equation AX=B, where a, b c in A and B are independent elements, X should be made of constants. Case 1 A=[a b 0;0 0 c]; B=[a b 0 0;0 0 c c]; The solution is X=[1 0 0 0;0 1...

16 February 2021 5,698 3 View

Alpha and dense granules in platelets not visible in Transmission Electron Microscopy?

I am trying to visualize alpha and dense granules in platelets. However, it seems like the granules were not stained well. Is there any way I can improve my staining technique to obtain better...

04 February 2021 5,925 9 View

How to improve my BrdU immunostaining?

24 January 2021 414 3 View

Just face a Comsol issue dealing with magnetically hyperelastic material?

Hello all, I am just newly doing a simulation to test the deformation status of a magnetized rubber-like material, which is in-compressible, under a shifting magnetic field. Try to find the...

22 January 2021 7,427 2 View

Any tips on Fab blocking of mouse tissue?

Hi, everyone, I am working on immunostaining of bone marrow and growth plates of mice. And I have to use an mouse monoclonal Ab to do this staining. However, with only secondary goat anti-mouse...

21 January 2021 9,826 3 View

NADP+/NADPH in bacterial cell lysates?

Hello all, we'd like to pick your brain . The enzyme we're testing requires NADPH to work. We have controls showing that our reaction needs our enzyme and our enzyme definitely needs NADPH. Since...

20 January 2021 232 2 View

Any suggestions on 50um cryosection immunostaining (growth plate) ?

Hi everyone I am working on immunofluorescence of cryosection of murine growth plates. The Ab worked well on 8um cryosection. But when I stained 50um section, I found the fluorescent signals...

03 January 2021 9,531 3 View

Do anyone have experience with zymo research yeast plasmid miniprep?

Hello, I am new to yeast research. I am trying to rescue plasmid from yeast and have tried several protocols developed by researchers. But the results are not promising. I ended up buying this kit...

06 December 2020 1,251 2 View

Why are all the cells are stained by EdU when performing EdU assay?

I was wondering whether there is anyone else have this problem? As shown in the images, almost all the cells are stained with EdU, some are whole nuclei (circle 1), some are puncta inside (circle...

02 December 2020 6,911 4 View

Would there loss some bases after the repairment to the viscous ends by using T4 DNA ligase?

In constructing shRNA plasmids, I want to shorten the size of the plasmids, so I want to use two restriction enzymes for restriction digestion, and then use T4 DNA ligase for complement and smooth...

25 November 2020 3,366 2 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

Do you consider undergoing a structure literature review / Meta analysis and getting numerical findings sort of quantitative method?

I am looking for a classification of the research methods included in literature review papers or Meta analysis ones .

22 February 2021 1,131 4 View

Can I do two kinds of mixed method in explanatory research?

Dear distinguish professors and scholars, I have a research in measuring two kinds: think tank capacity and research impact on policy. Can I do two kinds of mixed methods in order to find, "Is...

20 February 2021 6,897 1 View

How to analyse a (perceptual) study using differing/comparable sets of stimuli?

I plan on running an online perceptual experiment that has, let's say, 100 unique video stimuli altogether. Since these are too many to be rated by every participants, I would like to present the...

15 February 2021 7,444 2 View

How to analyse a split-plot design in stata ?

I have run an split-plot design experiment evaluating the efficacy of a treatment (with 3 whole plots and 4 sub-plots), and I am planning to analyse the collected data in Stata. However, I do...

14 February 2021 7,800 7 View

Can I use a likert survey for criterion sampling in a qualitative study or would that make it mixed methods?

I am doing a phenomenographic study on the reading experiences of avid v. Neutral v. Non-Readers. If I use a likert survey to criterion sample for participants, does that make it a mixed method...

09 February 2021 8,594 3 View

How to create the context-mechanism-outcome configuration (CMOc) in the realist methodology?

In the realist methodology (especially by Raymond Pawson et al), the CMOc is a common structure to explain what works, for whom, in what circumstances, and why? I'm always asked about the...

08 February 2021 2,792 1 View

Recommendations for sample size in visual qualitative research (using children's drawings)?

Hi everyone! Asking for a little help, if I may. I am working on a research project, where I aim to analyse children's drawings about Covid-19. I am fairly new to using visual data in qualitative...

07 February 2021 9,530 3 View

How do I create a categorical variable, level of exposure, and a dummy dichotomous, yes-no, variable for the Harvard Trauma Questionnaire Part 1?

I am using the Harvard Trauma Questionnaire (HTQ) Trauma Events scale (original version) that has 17 items (17 different traumatic events) with 4 response options, indicating 4 levels of exposure:...

04 February 2021 1,509 3 View

How to report path analysis / SEM with Yuan-Bentler correction from R output?

Dear all, I am currently writing my master thesis where I am analysizing my data using path analysis and SEM in R (lavaan package). Now that I like to write up my results, I am struggling with...

04 February 2021 8,993 2 View