What is your suggested method and materials for producing UV-curable transparent coatings?

What are the easy methods for synthesizing UV curable resins? I need to synthesis UV curable coatings. MMA seems to have good features for transparent coatings, but the additives and synthesizing...

24 April 2020 3,256 7 View

How can one learn about the effects of an organic substance on the human body?

For example, we synthesized organic matter called A, at this point we want to know its effects on the human body. I wanted to know if there was a special device for this or were they using other...

16 December 2019 2,223 3 View

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

In immunofluorescence assay, do we need to wash away (with 1X PBS) blocking buffer (10% FBS-PBS) before adding the first antibody?

25 September 2019 6,591 3 View

How can I attach an amine group (-NH2) to a carboxyl group (-COOH)?

20 September 2019 5,256 5 View

I need a method for plating nano-silicon on a metal substrate?

Coating of metals with silicon

13 August 2019 1,318 3 View

Is it possible to simulate hysteresis effect for piezoelectric material in COMSOL or ansys?

I tried sinusoidal input voltage with the amplitude of 100 V but couldn't get any hysteresis curve

24 May 2019 5,973 4 View

Taqman probe real time PCR...Problem regarding constructing plasmid dna standard curve?

Hi guys......i am facing problems regarding developing standard curves for different copies of plasmid DNA using taqman probe real time PCR. i have successfully obtained standard curve for...

06 March 2018 2,284 3 View

Plasmid DNA copy number dilution?

Hello everybody..... the concentration of my plasmid DNA is 126ng. i want to do real-time PCR taqman probe detection......i want to do serial dilution that should start from 10e8 copy number....so...

21 February 2018 3,230 3 View

Looking for a learning tutorial for TSOL software?

Hello I am trying to find a learning tutorial for TSOL software. I would be thankful if you help me in this way Best Regards Mehran Dehghan

27 October 2017 8,799 3 View

Is there any isoform of Rattus norvegicus TGF beta1 mRNA?

I want to do SYBR real time PCR for relative quantification of TGFB1. Gel electrophoresis of real time PCR product showed two bands in around about 80 bp (79 oC melting peak) and 180 bp (84 oC...

22 July 2017 2,265 1 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

Is there any tutorial for comsol general projections?

Hi, im trying to make a projection in a 3d comsol model. I have a non uniforme chanel and my intention is to get the average tempreture in the y-z cross section and se the average tempreture...

02 March 2021 4,624 4 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

Is there any expert in Smart PLS who can help me?

Dear colleagues, I have a manuscript that needs an expert in smart PLS to run the analysis, Is there anyone expert and ready to help me, please?

01 March 2021 8,212 3 View