Xanthomonadin is the pigment that is produced by the genus Xanthomonas. It is responsible for the yellow colour of the bacteria. This pigment has a major role in protecting the pathogen from harmful rays. The presence of the pigment is one of the confirmatory tests for the pathogen. When referring to the paper, the absorption maximum was between 441-444.7, but when I did, I got a peak at 447 and 448nm. How should I analyse this result?
Make sure about the culture viability and once again perform the experiment with same protocol, hopefully u can achieve your result.
All the best.............
Dear Sir thanks for your suggestion , But is culture viability plays a role with the pigment ? after 2 days of growth the pigment is extracted using solvent
As of you know pigments are photosensitive, design your protocol according to the desirable light conditions (take care of Incubation, Isolation of pigments, consistency with the solvent system).
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