In PRIMER, is it possible to downweight certain taxa in multivariate community datasets to reflect uncertainty around identification??

I have a dataset with about 80 different species. As usual, some species are very easy to identify with certainty whereas others are more difficult, which means that I am less certain of my...

03 March 2021 8,066 4 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

OpenBCI eeg--has anyone used them?

I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...

01 March 2021 4,128 3 View

How to remove DCU and HOBT from my reaction?

I am doing a acid and a chiral amino alcohol reaction using DCC and HOBT in DCM or THF? Can anyone suggest me how to remove DCU and HOBT from a reaction mixture??

28 February 2021 2,153 1 View

1 nm thick MgO thin-film to be deposited on Si wafer using thermal evaporation. What will be the optimum deposition conditions?

Favourable conditions of base pressure, rate of deposition. Also, by which characterisation technique to be used to verify the thickness of the deposited film.

28 February 2021 3,640 3 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

What is the suitable range of Sulfur content and Viscosity of Heavy fluid Oil that used in Kiln?

What is the disadvantages of high sulfur content to the ceramic burning process? and what is the effect of outranged viscosity?

24 February 2021 8,219 2 View

How to empty large intestine in mice ?

Hi all I am working on colon-targeted drug delivery for which I kept mice fasting for 6h using fasting grills during scotophase (dark period). I observed liquid fecal content in the cecum and...

23 February 2021 7,701 2 View

Does anyone know how to isolation of high-purity myenteric plexus from adult rat gastrointestinal tract for further biochemical investigation?

I would like to isolate myenteric plexus from the rats small intestine and then isolate certains components of ENS (enteric nervous system) by biochemical tests.

04 February 2021 6,068 2 View

Can Fecal Microbiota Transplantation from young heathy person to elderly persons improve health conditions?

06 January 2021 7,353 10 View

Why are the organoid cells showing blue fluorescence even without dapi stain?

Mouse intestinal organoids ; After fixation and storage in PBS-T, when I checked in microscope, the organoid show fluorescent color blue even if I have not added DAPI. Does anyone have such...

05 January 2021 2,067 3 View

Can anyone tell me the name of these parasites which I got from a marine fish?

I got these parasites from eye and intestine of a marine fish. Thank you.

09 December 2020 7,752 2 View

Is it alright to reuse the MinION flow cells using the same set of barcodes but for a different type of approach/targets?

It's generally recommended to use a different set of barcode when reusing flow cells due to issues of barcode carry over despite thorough washing of the flow cell when running another batch of...

05 December 2020 5,544 1 View

How to estimate the effect of nematodes on some varieties of wheat?what are the correlation possible to do ?

Hi , I carried out a test on the behavior of some varieties of durum wheat with respect to the CCNs, two successive years, in pots, and I inoculated with the same number of cysts (Pi), at the end...

05 December 2020 7,187 3 View

What is the best way to filter out cell debris/small particles from a cell prep?

Hello, I am isolating lamina propria cells from intestinal tissues and I would like to add an extra step at the end to filter out cell debris but preserve my cells. Should I use a 22um filter and...

14 October 2020 3,266 2 View

How to obtain good cryosections of mouse intestine?

I am attempting to do mouse intestine longitudinal cryosections, but the sections after hematoxylin and eosin staining are not at their best (the villi keep breaking apart). My protocol: I rinse...

14 October 2020 8,432 3 View

Can anyone help me in doing whole mount immunostaining on mouse colon?

I am exactly looking forward to know the procedure to isolate the myenteric and submucosal layer from intestinal whole mount. I would like to see the degeneration of dopaminergic neurons on it?...

12 October 2020 6,069 2 View