I am going to prepare titanium nanoparticles using plant extract as reducing agent. I found from differnt research papers. All have different methods. Like one paper saying after mixing Titanium salt and plant extract put it for 24 hours until the appearance of green color which is the indication of TiO2 nanoparticles. Then go for centrifugation and calcination of particles in muffle furnace for 3 hours.Another author saying combine plant extract and Ti salt and stirr it for 15 mins. After that make its pH upto 8 and filter the precipitates, dry them and put them for calcination in muffle furnace.Is the appearance of green color necessary for the indication of titanium oxide nanoparticles?