I am doing undergraduate research for Mushroom growth-enhancing using Bacteria.
Hello dear scientists How can we distinguish maternal contamination from triploidy in QF-PCR analysis?
09 December 2023 9,519 2 View
Hello dear scientists. Can the specificity of primers change over time or depending on the sample? It looks like the primers I used in my recent PCR to target exon13 of CFTR gene on chromosome 7,...
22 August 2023 9,887 5 View
Hello dear scientists Where can I find the Liz 1200 size standard for Gene marker software ?
18 July 2023 1,407 1 View
Hello dear scientists Ideas for good articles or databases on cystic fibrosis and common or potentially pathogenic variants are most welcome. And what strategies would you suggest for carrier...
22 June 2023 2,316 4 View
Hi everyone! I'm wondering if anyone here has any experience with the Dynabeads Protein G immunoprecipitation kit (#10007D) from Invitrogen. I'm working on a project that involves...
13 June 2023 4,974 5 View
Hello, dear researchers I want to set up a test to diagnose Huntington's disease. I would appreciate any guidance on the database where I can find new standards and approved protocols for genetic...
31 March 2023 835 1 View
As mentioned in the title, I can't find the protocol of using DSS to do crosslinking dynabead protein G with antibody on the Internet. Can anyone who has experience give me some tips, thank you...
12 March 2023 4,489 0 View
How do I calculate the organic loading in kg VS m-3? I'm examining the amount of methane produced from anaerobic digestion. This was carried out in 125 ml serum bottle with a working volume of...
30 June 2022 8,607 0 View
Hello, I'm a student research looking to apply a multiple linear regression to my data set of all continuous values. My question is mainly about a step-by-step idea of how to do this, as I believe...
28 May 2022 587 3 View
In supine surgery, free-running EMG is often not observed, although the patient is observed to move. Is this possible?...
31 May 2021 1,273 5 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I understand a glycerol stock for long-term preservation would be ideal, but how are working cultures generally stored? Mainly concerning Candida spp. isolates. A few references mention that they...
22 July 2024 6,592 3 View
Greetings: I am looking to buy/obtain the auxotrophic strain Aspergillus oryzae NSAR1, but I just have found one culture collection from Japan but haven't received any answer. Could you provide...
11 July 2024 6,847 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,251 1 View
From my knowledge- mushrooms are highly sensitive to temperature. How can growers manage suitable temperatures?
22 June 2024 7,346 0 View
Hola a todos. Me gustaría saber si alguien conoce algún documento de consulta de claves dicotomicas - taxonómica para hongos microscópicos, especialmente que me pueda ayudar a determinar especies...
15 June 2024 6,693 0 View
Hello I would like if you know some document for search cheats dichotomy - taxonomy for fungus microscopy, for example if you could help me to identify some kind of Beauveria, Penicillium y...
15 June 2024 1,289 0 View
soil fungal diversity of hevea brasiliensis
14 June 2024 9,389 0 View
I want to design a study for ex situ conservation of rare ferns based on ecological and genetic diversity. what could be possible method other than cryopreservation of spore for a short term...
10 June 2024 4,992 1 View
I am using CTAB method (Naser M. Salameh et al., 2014) for fungal DNA isolation and purification. During reagents preparation I am not sure weather I should autoclave 2X CTAB and Wash buffer. So...
09 June 2024 8,470 2 View
