What is the best way to isolate extracellular vesicles from cell culture media if they are infected with viruses?
I am talking about an experiment in which we want to study the components inside the exosome after viral infection. I want to make sure that the isolation is clean of viruses.
If you mention the kit and the solutions, I will be grateful to you.
Hey Faisal,
Maybe you could try an approach that targets surface markers of extracellular vesicles. I attached a protocol for an affinity chromatography method. For more details regarding the unlerlying technology, you can have a look here: https://www.iba-lifesciences.com/strep-tag/exosome-isolation/
Here you can find some application examples: https://www.iba-lifesciences.com/resources/application-examples/#nav-exosome-isolation
Best,
Alex