I am looking for some information regarding innate lymphoid cells isolation and characterization from mouse gut. Best markers and techniques to have those cells. Thank you in advance.
Hello
Timotheus and et al see the multicolor flow cytometry is the only reliable method to purify and characterize ILCs . This is due to the presence of three groups namely ILC1, ILC2, and ILC3, based on the cytokines that they produce. also , They comprise less than 1% of lymphocytes in mucosal tissues and express no unique cell surface markers. Therefore, they can only be identified by combinations of multiple cell surface markers and further characterized by cytokine production in vitro.
see full text of this paper in one of this link s
http://onlinelibrary.wiley.com/doi/10.1002/0471142735.im0325s106/abstract
OR
https://www.researchgate.net/publication/264431713_Isolation_and_characterization_of_mouse_innate_lymphoid_cells
Good luck
http://www.hindawi.com/journals/mi/2014/235460/
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3699860/
Article Isolation and Characterization of Mouse Innate Lymphoid Cells
You can please follow the links below to obtain certain interesting informations:-
1. http://onlinelibrary.wiley.com/doi/10.1002/0471142735.im0325s106/abstract
2. http://www.pubfacts.com/detail/25081911/Isolation-and-characterization-of-mouse-innate-lymphoid-cells
3. http://www.nature.com/nprot/journal/v10/n5/abs/nprot.2015.047.html
you can check my answer to other question. Of course that depends on how many colors you have in the flow cytometry, we have a 10 color flow, so I am using more markers to stain ILCs
https://www.researchgate.net/post/What_is_the_best_gating_strategy_six_color_max_for_analyzing_all_types_innate_lymphoid_cells
Hi,
do you know if there is any reliable staining to characterize ILC by confocal microscopy on human gut biopsie?
thanks
stefania
Hello,
STEMCELL Technologies has a kit that can pre-enrich for ILC2s from mouse lung tissue by immunomagnetic negative selection. The protocol only takes 16 minutes once you have a single cell suspension. In naive mouse lung samples, you usually get a 7-fold enrichment. Pre-enriching significantly reduces the time you need on a sorter so it's definitely worth considering. It was used in the Immunity publication attached.
It should work for other tissues as well (ie. gut or tonsil) but might need some optimization.
Good luck,
Jeff
Article Allergen-Experienced Group 2 Innate Lymphoid Cells Acquire M...
- Badges
- Science topic
- Similar topics
- Biological Science
- Immunology
- Immunology Techniques