Dear Scientists

We are working on in vivo anticancer activity of natural products. We want to induce hepatic cancer using N-nitrosodiethylamine obtain from Sigma compagny.

1- What is the best protocol to induce this pathology?

2- We were expected N-nitrosodiethylamine in a solid form, but it was a solution. Is there other form of this reagent?

3- How to obtain different doses for induction of this pathology with N-nitrosodiethylamine solution?

Thanks