How to use less antibody in a Western blot?

I'm trying to figure out how to not need so much antibody for our Western blots. Right now, we use 10 mL of buffer to dilute our antibodies in. Any less than that, and the membrane isn't submerged...

27 August 2019 8,607 3 View

Can you help me to create a fuzzy model for interaction IoT devices?

I have probleme to build a fuzzy model/controller for organisation interaction devices in IoT network!

18 June 2019 2,681 3 View

Primer Concentration of RT?

Hello, I am using a gene speciic primer to perform reverse transcription. I need to use 20pmols of my primer in a final reaction volume of 20ul. Is 20pmol interpreted as 20pmol/uL (20uM) in...

28 October 2018 7,946 4 View

What is the relationship between Intelligence and Learning?

Describe the relationship between intelligence and learning? May you please provide journals or articles about this matter. Thanks!

13 June 2018 2,441 8 View

Are there limitations when using AMOS or the PROCESS macro for SPSS to test for moderation?

I've often heard that there is virtually no reason why someone would prefer using AMOS to test for moderation than PROCESS and that it is overall more troublesome. Some even argue that PROCESS is...

01 November 2015 1,282 16 View

How can I compute risk differences and risk ratio estimates from a zero-inflated negative binomial regression for both parts of the model, using SAS?

18 October 2015 9,249 1 View

Wich protocol are you using to stain frozen liver sections with oil red o ?

I have tried till today a lot of protocols (IHC world,...), using isopropanol or propylene glycol or both, but I haven't had any satisfaying results yet...The stain is scattering everywhere out of...

07 September 2015 6,449 7 View

Why do I see "filaments" in my adhesion assays ?

04 March 2015 7,209 2 View

Can someone please help me understand some aspects of conducting a factor analysis please?

I have collected my data and I am now analyzing it. Here are the areas I cannot understand: 1. The pre-post tests questions are alike every way (except in how participants respond after exposure...

18 October 2014 9,540 7 View

Is there any difference in teaching in a University to teaching in a Community College?

I'm being asked if I've ever taught in a Community College as a precursor to a learning/design discussion. There seems to be a belief that teaching and learning in Community Colleges today, are...

30 April 2014 6,878 9 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What is the exact mechanism of the antimicrobial effect of local anesthetics?

The antimicrobial effect of local anesthetics has been known for more than 50 years, but it still seems to have an unclear mechanism of action. which bacteria? which concentration? etc etc. ....

27 February 2021 4,450 2 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

Uncertianiny in using dry or wet basis in mixing a sediment with fuel oil?

Hello I'm currently working on a topic related to studying the anaerobic biodegradation of TPH in marine sediments. In this work, sediment should be mixed with fuel oil to a concentration of 20 g...

24 February 2021 9,839 3 View

What are the pros and cons for mice versus rats?

Hi Everyone, I am designing a pilot study using rodents to assess the effects of simulated space environments (radiation + altered gravity) on microbiome and immune system. I have limited...

24 February 2021 1,825 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View