How to take XRD of colloidal Ag NPs?

I have synthesized silver nanoparticles using sodium citrate as reducing agent. I have carried out XRD characterization by drop coating colloidal solution on the glass substrate ( since yield in...

27 January 2021 1,416 3 View

CFD steady state simulation not converging even after large number of iterations for multiphase flow?

Dear All, I have been working on multiphase flow through a complex geometry having porosity 0.25. I have patched it with oil and high-salinity brine. At the inlet I am flowing low-salinity brine....

04 December 2020 4,506 3 View

Absolute values in Leptin ELISA kit are higher than normal. Does it depend on washing procedure?

I have been using mouse leptin ELISA kit (Catalog number (90030) from crystal chem. I have been doing manual washing between my steps as I didn't have automatic washer instrument. Does this cause...

12 August 2020 4,352 4 View

Which book/study material would be best for a beginner in optimization research field ?

power system optimization problems and optimized unit commitment

20 April 2020 6,248 12 View

How to generate a magnetic field around a sand packed column?

Hi all! I want to make a lab scale setup. I want to generate a magnetic field around a sand packed column. I want to inject magnetic nanoparticles in th sand column and want to track them...

07 April 2020 1,393 1 View

Is it possible to remotely detecting location of fluorescent nanoparticles in a core sample using a quencher?

Dear all, I have injected fluorescent-based nanoparticle fluids in core samples. Is it possible to remotely detect the location of fluorescent nanoparticles in a core sample using a quencher? Thanks

11 January 2020 3,102 3 View

Should oil recovered after water flooding be similar for all core flooding experiments to compare oil recovered by different surfactants in CEOR?

I am performing core flooding experiments to compare percentage of additional oil recovered during surfactant flooding in a sand pack column. Is it necessary that after secondary waterflooding...

16 December 2019 2,886 2 View

Is it possible to get the two laser spots produced by using a wedge prism in a single line ?

I split the laser light into two parts by using a wedge prism, but the two spots are not in a single line. How can I make it in a single line? Is it possible to increase the separation between the...

03 December 2019 7,168 3 View

Is it possible to split a laser light (1064 nm) into two equal parts by using wedge prism? Any other optics required along with wedge prism?

I have tried to split the 1064 laser light into two equal energy spots by using a wedge prism of wedge angle 2 degree. But I cannot split the laser light. There are so many papers reported the...

12 November 2019 7,645 3 View

For addressing the present research question, using thematic analysis, must I aim for an equal sample size?

Dear fellow researchers, I am a novice when it comes to qualitative methodology. Although I have gone through the article of Braun and Clarke (2006), and referred to a number of other resources,...

09 November 2019 300 8 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View