What is the best way to dissolve the substrate chitin-azure (Sigma) to do chitinase assays?

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Karl Rumbold

you don't dissolve the substrate! Details on the assay can be found in Somers et al., 1987.

Marieta Cawood

Thank's very much Karl

Ashok Kumar Nadda

These articles will be helpful for your study.

Please see

https://www.researchgate.net/publication/311668087_Improvement_of_chitinase_Pachi_with_nematicidal_activities_by_random_mutagenesis

https://www.researchgate.net/publication/282153514_Enhanced_nematicidal_potential_of_the_chitinase_pachi_from_Pseudomonas_aeruginosa_in_association_with_Cry21Aa

https://www.researchgate.net/publication/275278845_Effects_of_domains_modification_on_the_catalytic_potential_of_chitinase_from_Pseudomonas_aeruginosa

Thanks regards

Article Improvement of chitinase Pachi with nematicidal activities b...

Article Enhanced nematicidal potential of the chitinase pachi from P...

Article Effects of domains modification on the catalytic potential o...