I am working on the disruption of some yeast genes to create new strains... for my research project "Metabolic Engineering of Saccharomyces cerevisiae as lipid cell factories for Bio-Diesel production"
While you can use point mutations to introduce premature stop codons close to the start of the open reading frame, this would leave room for revertants to occur. As such, deletion of the genes in question is safer. See Chapter Gene Disruption in the Budding Yeast Saccharomyces cerevisiae
Check https://scholar.google.ch/scholar?as_ylo=2020&q=yeast+gene+disruption&hl=en&as_sdt=0,5&as_vis=1 for more recent examples