What is the best way for peripheral blood leukocytes storage for long period of time (15 months) ?

More Farzin Beglari's questions See All

Waffle slabs for 5-7-meter span?

In my city, 1 and 2way waffle slabs are used for residental houses with 5-7-meter span. As I know waffle slabs are suitable and economical for the span with more than 9 meters. Is it economical...

06 February 2024 8,782 0 View

What is the lowest concentration (either titrant or titrate) that can be used for a Nano ITC instrument?

I am trying to perform isothermal titration calorimetry (ITC) for quantifying DNA and liposome binding thermodynamics, but the samples are in low volume and very low concentration (~40 nM). Do you...

07 August 2023 3,615 1 View

What would be the best choice to block the cells described below?

Hello everyone, I have a question about flow cytometry and its procedure in order to analyze cell-surface markers. What would be the best blocker while the cell line and secondary antibody are...

17 August 2022 2,493 0 View

How can i get rid of Primer Dimer (PD) formation?

Hello I am working with Real Time PCR, some how in some patients i gain melting curve same as attached file. How can i eliminate primer dimer formation. 1. I reduce primer concentration, but i...

14 January 2022 7,890 3 View

Is there any contactless method to measure the internal quantum efficiency of a junction?

I have a simple pn junction without any contact layer, and I want to measure the quantum efficiency of the junction.

31 October 2021 8,951 3 View

Troubleshooting of qRT-PCR with out any curves?

I extracted total RNA from blood leukocytes and produced cDNA from 2ug of RNA, which assessed cDNAs integrity and annealing temperature of primers by electrophoresis. In following steps, for...

13 October 2021 8,527 2 View

What are the appropriate methods for scenario reduction?

I am looking for a good method to reduce the number of scenarios in problems with a large number of uncertainty parameters. I implemented some of these methods in MATLAB, but unfortunately the...

23 August 2021 7,554 3 View

Introducing methods to deal with uncertainties in power systems?

Please introduce a suitable resource for teaching methods to deal with uncertainties in power systems. (For example, Monte Carlo method, fuzzy, robust, IGDT, etc.) The suggested source should...

28 December 2020 9,908 3 View

Does anyone know about the importing topography lines from autocad to MIKE3?

I want to import the topography auto cad file to mike 3. could you please guide me?

12 March 2019 9,885 1 View

Where can I find a "riot" or "protest" related dataset of tweets?

I already have a dataset about the 2011 England riots, but the dataset is collected between 09 Aug. to 26 Aug. However, the riots took place between 06 Aug. until 10 Aug. In addition to that, I...

11 February 2019 9,448 2 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

How to Compress Information Neurally?

Samuel Morse, the inventor of the Morse Code, understood that certain letters in the English language occurred more frequently than others (Gallistel and King 2010). To deal with this, Morse used...

01 August 2024 4,456 2 View

Where to find a gene list for CRISPRa/i library screening of regulatory factors that affect pathogenic Th17 differentiation in PBMC?

I want to perform a CRISPRa/i library screening for candidate regulatory factors that affect pathogenic Th17 differentiation in PBMC. But I am wondering how to define the size of gene library, so...

31 July 2024 2,150 0 View

Can you visualize platelets using EVOS ?

Hi all, My lab has Thermo Scientific™ Invitrogen™ EVOS™ FL Auto 2 Imaging System, and I was wondering if I will be able to use it with whole blood, whilst focusing on platelets? The idea would...

24 July 2024 9,337 3 View

Culture plates for PBMC (non-adherent). For infection?

Hello, good morning. I would like to know which plate I can use to infect fresh PBMC with virus and culture for 24 hours. I have tried using 12-well and 24-well plates, but many cells adhere and...

24 July 2024 2,158 2 View

Why am I observing very high Temperature and Pressure Increase using NPT Ensemble?

Hello everyone, I am running DFT with CP2K software at 1200 K, 1 bar with a periodic system of 64 Al atoms + 2 CO2 atoms. However, I am seeing my temperature slowly increase to >4000 K and my...

22 July 2024 3,177 0 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

How can we improve storage conditions of museum objects?

90% of museum objects worldwide are in storage.

21 July 2024 8,038 6 View

Detaching human-derived monocytes?

Hi, I need help in detaching adherent monocytes from well plates. I isolated the PBMCs from buffy coat and I did CD14+ positive selection to collect only the monocytes. I let them adhere on my...

20 July 2024 7,922 2 View

Prof. Ayad Ghany Ismaeel

Hi Colleague

Find the following URL may help you:

Article High DNA stability in white blood cells and buffy coat lysat...

Regards...

Alexandr Chernov

Hello, Farsin!

Look at following publication, please:

Article Investigating gene expression profiles of whole blood and pe...

Farzin Beglari

Thank you so much Dr🙏