Farzin Beglari

3 Questions 3 Answers 0 Followers

Questions related from Farzin Beglari

How can i get rid of Primer Dimer (PD) formation?

Hello I am working with Real Time PCR, some how in some patients i gain melting curve same as attached file. How can i eliminate primer dimer formation. 1. I reduce primer concentration, but i...

15 January 2022 7,916 3 View

Troubleshooting of qRT-PCR with out any curves?

I extracted total RNA from blood leukocytes and produced cDNA from 2ug of RNA, which assessed cDNAs integrity and annealing temperature of primers by electrophoresis. In following steps, for...

14 October 2021 8,555 2 View

What is the best way for peripheral blood leukocytes storage for long period of time (15 months) ?

hello I want to know that what is the best way for storage blood leukocytes to prevent gene expression profiles (mRNA) for long time. is cryopreservation appropriate or whole blood is...

05 February 2021 6,467 3 View