Some kits like QIAEX II Gel Extraction Kit are used to obtain bands from DGGE gels, but also some researchers only eluted the fragment in water, how to get the best results?
I have only used the water method-it works well as long as you get defined banding in your gel and good separation. I just stabbed the band with a sterile tip, placed it into 50 uL of sterile water and mixed it on a vortex briefly. then leave overnight and use the water as a template for PCR..