Dear all
I have tried 1ml Trizol to extract total RNA from crabs gills. The weight of most of my samples is + -0,03 g. We have applied this protocol for hepatopancreas samples and the results were very good. However, for gills, the efficience of RNA extraction was ok for less than 25% of samples. We are using petles for microtubes (RNAse free).
Any information you can provide me would be greatly appreciate.
Cheers
Isabella