We have a Crude extract made up of Garlic Peels and we want to dilute it using a solvent to create concentrations. We don’t know what is the best and capable for our extract.
I recently isolated mice cardiomyocytes and found some are not individual cells. I tried to stain the cells with Wheat Germ Agulutinin, it did not work. Does anyone have recommendations on the...
20 June 2024 3,225 2 View
Could you advise me on a simple technic for DNA sequence & termination definition (characterization) ? (I am non-biologist). Which parameters should I take in consideration ? Thank you
26 May 2024 1,590 3 View
I try to understand and identify my dsDNA structure. I need to know if my DNA has a special structure and it is double-stranded or not. Which method should I use ? Are FTIR and CD sufficient ? I...
26 May 2024 5,696 1 View
Hello everyone, I'm doing my master's thesis right now and planning to use Response Surface Modeling. The plan is to test how the (in-)congruence of a personality trait of two people affects...
20 May 2024 9,811 0 View
Nutrient recovery is becoming the order of the day as purchasing NPK has become more costly lately. In the advent of food security issues, one is tempted to get cheaper means to produce nutrients...
15 May 2024 767 1 View
For example: If the data is a song. Is the sentence analysis a line-by-line?
28 April 2024 7,251 0 View
Hello Everyone! I use Golden Gate technology to build plasmids in my lab. I have a plasmid that I've built that I'm now trying to maxi prep. I've already done a miniprep on this plasmid and sent...
22 April 2024 6,700 8 View
This question is about the article "Tenets of Specimen Management in Diagnostic Microbiology," written by Rajeshwar Reddy Kasarla and Laxmi Pathak in 2023. The question centers on the advantages...
10 April 2024 2,118 2 View
According to the study "The transition from gel separatory serum tubes to lithium heparin gel tubes in the clinical laboratory", most clinical chemistry and immunoassay tests can be performed...
09 April 2024 6,395 1 View
I cultivate CAF from the specimen of the pancreatic cancer patient who had pancreaticoduodenectomy (PD). Other than the medium, FBS, antibiotics, is EGFR needed for the culture?
07 April 2024 8,937 0 View
I need to do some experiment with it in liquid form.
07 August 2024 613 3 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View
Why methanol and sulphuric acid, used in the analysis of polyhydroxyalkanoates (PHA) methyl esters by GC-MS? Additionally, why do we typically use non-polar solvents in GC-MS?
22 July 2024 1,210 2 View
Is it the best optimization ratio based on experiments?
22 July 2024 8,170 0 View
If you don't understand my question let me know
21 July 2024 563 1 View
I used several solvents and measured the absorbency and determined the total phenolic content, but the results were similar after dropping them on the titration curve; What is the reason, please help
17 July 2024 5,151 5 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View
I synthesised a nanocarrier by conjugation MOF (MIL-100) with folic acid for use in chemotherapy but the samples didn't dissolve completely in aqueous solution or DMSO. Instead, they only...
15 July 2024 2,414 1 View
as of my knowledge z90 is acrylic based sealant adhesive.
09 July 2024 8,660 4 View