What is the best solvent system to run methanol extract on TLC
Hi, please search in the Camag TLC data base, just insert the plant's name, maybe you can find some publications, so you can consider to apply the published methods for your work.
http://www.camag.com/en/tlc_hptlc/customer_magazine_cbs/ccbs_database.cfm
Chloroform:methanol combinations (7:3; 8:2; 9:1 and other variants) would be useful for methanolic extract in most of the cases.
Toluene : Chloroform : acetone (7:5:8 v/v) would be useful for methanol extraction, due to co-solubility of moderately polar, polar component are drag out in similar-polarity ( methanol) component. In most of cases some secondary plant metabolite (flavonoid, polyphenol,etc ) are extracted out by methanol. If you use that solvent along with chamber saturation, relative humidity, etc consideration you may good resolution RF in 0.3 -0.8 .
Salem, If the sationary phase is silica gel and the products in the extract are very polar mixtures of CH2Cl2 and MeOH do not give good separation. Try ternary or quaternary systems of eluents such as : CHCl3-MeOH-Water 5/4/1 v/v/v or CHCl3-MeOH-Water-Acetic Acid 5/4/0.5/0.5 v/v/v/v.
meoh:acetic acid:water in a ratio 8:1.5:0.5 used this solvent combination may be help you.......
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